One challenge in point-of-care (POC) diagnostics is the lack of room-temperature methods for RNA detection based on enzymatic amplification and visualization steps. Here we perform reverse transcription lesion-induced DNA (RT-LIDA), an isothermal method that only requires T4 ligase. RT-LIDA involves RNA-templated ligation primers to form complementary (cDNA) followed by toehold-mediated strand ...

  Objective(s): Hypoxia is a serious challenge for treatment of solid tumors. This condition has been manifested to exert significant therapeutic effects on glioblastoma multiform or (WHO) astrocytoma grade IV. Hypoxia contributes numerous changes in cellular mechanisms such as angiogenesis, metastasis and apoptosis evasion. Furthermore, in molecular level, hypoxia can cause induction of DNA br...

Background: IFN-g is mostly secreted by activated CD4+ , CD8+ T cells and NK cells. This cytokine has immunomodulatory, anti-cancer and anti-microbial effects and is important for prophylaxis, diagnosis and treatment of chronic infections and cancers. Objective: The purpose of this study was to clone the full cDNA of human IFN-g and express it in CHO cell line. Methods: Lymphocytes from a healt...

smart card abstract pdf Smart cards are used in information technologies as portable integrated.Abstract: While mobile handheld devices provide productivity benefits, they also pose. Paper describes two novel types of smart card with unconventional form factors. smart card abstract ppt Pdf.ABSTRACT Over the past few years, smart cards have achieved a growing acceptance as a powerful tool for se...

Most cDNA library screening procedures do not distinguish between full-length and incomplete clones and therefore may yield incomplete cDNA fragments. Thus, there is a widespread need for a method allowing the efficient selection of full-length clones. I present a rapid, PCR-based method that allows the simultaneous screening of > 10(6) cDNAs. The longest cDNA is identified in the first step so...

These days, unless something has the epithet “smart” attached to it, it is nothing. Smart Energy solutions promise cleaner, cheaper and more reliable energy. Smart Cities promise better quality of life for its citizens. We will argue that for a “system” to be SMART, it should Sense Meaningfully, Analyze and Respond Timely. Using real-world examples from the domains of Smart Energy and Smart Cit...

The Internet of Things (IoT) has recently emerged as enabling technology for the smart gird, smart health, smart transportation, and smart environment as well as for smart cities. The major smart grid devices are smart home appliances, distributed renewable energy resources and power substations. The seven domains existing smart grid conceptual model was developed without the IoT concept in min...

Smart Classroom is a Smart Space developed to enhance traditional tele-education practice. This paper describes the considerations of bringing forward Smart Platform as the software infrastructure of Smart Classroom system, abandoning the former implementation based on OAA. As a multiagent system for Smart Space, Smart Platform encompasses following features: spontaneous discovery of runtime en...

Smart camera networks are real-time distributed embedded systems that perform computer vision using multiple cameras. This new approach has emerged thanks to a confluence of simultaneous advances in four key disciplines: computer vision, image sensors, embedded computing, and sensor networks. In this chapter, we briefly review and classify smart camera platforms and networks into single smart c...