Life-cycle assessment (LCA) is an environmental assessment tool that quantifies the environmental impact associated with a product or a process (e.g., water consumption, energy requirements, and solid waste generation). While LCA is a standard approach in many commercial industries, its application has not been exploited widely in the bioprocessing sector. To contribute toward the design of mor...

Heat generation is a very conspicuous phenomenon in large scale bioreactors and could in principle quite easily be measured by the temperature increase of the cooling water. Taking yeast cultures growing on a mixed oxido-reductive metabolism for various reasons as an example, this paper demonstrates how heat dissipation measurements may be used in combination with one other on-line measurement ...

Alcaligenes latus has been known to produce poly(3-hydroxybutyrate) (PHB) in a growth-associated manner even under nutrient-sufficient conditions. However, the PHB content obtained by fed-batch culture was always low, at ca. 50%, which makes the recovery process inefficient. In this study, the effect of applying nitrogen limitation on the production of PHB by A. latus was examined. In flask and...

While the nutrient limited fed-batch technology is the standard of the cultivation of microorganisms and production of heterologous proteins in industry, despite its advantages in view of metabolic control and high cell density growth, shaken batch cultures are still the standard for protein production and expression screening in molecular biology and biochemistry laboratories. This is due to t...

Environ. Entomol. 22(2): 432-437 (1993) ABSTRACT Bacillus thuringiensis Berliner toxicity is a dose-dependent phenomenon, and the dose acquired by an individual is directly related to the quantity of treated food consumed. A bioassay was conducted using two Apium graveolens var. rapaceum (L.) cultivars to determine if the B. thuringiensis dose acquired by Spodoptera exigua (Hiibner) (Lepidopter...

Doubly fed induction generator (DFIG) wind power generation system is widely used in farm all over the world. Reactive can be generated both grid-side converter and generator-side of DFIG. In this paper, working principle control method DFIG are introduced, reactive limit derived, finally regulation simulated Simulink.

A dynamic model of glucose overflow metabolism in batch and fed-batch cultivations of Escherichia coli W3110 under fully aerobic conditions is presented. Simulation based on the model describes cell growth, respiration, and acetate formation as well as acetate reconsumption during batch cultures, the transition of batch to fed-batch culture, and fed-batch cultures. E. coli excreted acetate only...

BACKGROUND Fungal morphology and aeration play a significant role in the growth process of Mortierella alpina. The production of microbial oil rich in arachidonic acid (ARA) in M. alpina was enhanced by using a multi-stage fermentation strategy which combined fed-batch culture with precise control of aeration and agitation rates at proper times. RESULTS The fermentation period was divided int...

With the remarkable increase of genomic sequence data of microorganisms, novel tools are needed for comprehensive analyses of the big sequence data available. The self-organizing map (SOM) is an effective tool for clustering and visualizing high-dimensional data, such as oligonucleotide composition on one map. By modifying the conventional SOM, we developed batch-learning SOM (BLSOM), which all...

[This corrects the article DOI: 10.1186/s13068-016-0466-5.].