نتایج جستجو برای: shotgun

تعداد نتایج: 3944  

Journal: :Chemistry & biology 2003
Katsuyuki Murase Kim L Morrison Phillip Y Tam Ryan L Stafford Frances Jurnak Gregory A Weiss

The highly abundant GTP binding protein elongation factor Tu (EF-Tu) fulfills multiple roles in bacterial protein biosynthesis. Phage-displayed peptides with high affinity for EF-Tu were selected from a library of approximately 4.7 x 10(11) different peptides. The lack of sequence homology among the identified EF-Tu ligands demonstrates promiscuous peptide binding by EF-Tu. Homolog shotgun scan...

2002
Michael F. Kim

The motivation for sequencing genomes is rather direct. According to the central dogma of biology, elements of the genome are translated via several mechanisms into the structure and function of organisms. But in order to study genomes, we must first sequence them. Whole Genome Shotgun (WGS) is a successful method for sequencing genomes. This lecture we will discuss how WGS works, some of the c...

2015
E. Doster P. Rovira N. R. Noyes B. A. Burgess X. Yang M. Weinroth L. Linke R. Magnuson K. Jones C. Boucher J. Ruiz P. S. Morley K. E. Belk

Journal: :Advances in Computers 2004
Mihai Pop

Shotgun sequencing is the most widely used technique for determining the DNA sequence of organisms. It involves breaking up the DNA into many small pieces that can be read by automated sequencing machines, then piecing together the original genome using specialized software programs called assemblers. Due to the large amounts of data being generated and to the complex structure of most organism...

Journal: :Science 2000
M D Adams S E Celniker R A Holt C A Evans J D Gocayne P G Amanatides S E Scherer P W Li R A Hoskins R F Galle R A George S E Lewis S Richards M Ashburner S N Henderson G G Sutton J R Wortman M D Yandell Q Zhang L X Chen R C Brandon Y H Rogers R G Blazej M Champe B D Pfeiffer K H Wan C Doyle E G Baxter G Helt C R Nelson G L Gabor J F Abril A Agbayani H J An C Andrews-Pfannkoch D Baldwin R M Ballew A Basu J Baxendale L Bayraktaroglu E M Beasley K Y Beeson P V Benos B P Berman D Bhandari S Bolshakov D Borkova M R Botchan J Bouck P Brokstein P Brottier K C Burtis D A Busam H Butler E Cadieu A Center I Chandra J M Cherry S Cawley C Dahlke L B Davenport P Davies B de Pablos A Delcher Z Deng A D Mays I Dew S M Dietz K Dodson L E Doup M Downes S Dugan-Rocha B C Dunkov P Dunn K J Durbin C C Evangelista C Ferraz S Ferriera W Fleischmann C Fosler A E Gabrielian N S Garg W M Gelbart K Glasser A Glodek F Gong J H Gorrell Z Gu P Guan M Harris N L Harris D Harvey T J Heiman J R Hernandez J Houck D Hostin K A Houston T J Howland M H Wei C Ibegwam M Jalali F Kalush G H Karpen Z Ke J A Kennison K A Ketchum B E Kimmel C D Kodira C Kraft S Kravitz D Kulp Z Lai P Lasko Y Lei A A Levitsky J Li Z Li Y Liang X Lin X Liu B Mattei T C McIntosh M P McLeod D McPherson G Merkulov N V Milshina C Mobarry J Morris A Moshrefi S M Mount M Moy B Murphy L Murphy D M Muzny D L Nelson D R Nelson K A Nelson K Nixon D R Nusskern J M Pacleb M Palazzolo G S Pittman S Pan J Pollard V Puri M G Reese K Reinert K Remington R D Saunders F Scheeler H Shen B C Shue I Sidén-Kiamos M Simpson M P Skupski T Smith E Spier A C Spradling M Stapleton R Strong E Sun R Svirskas C Tector R Turner E Venter A H Wang X Wang Z Y Wang D A Wassarman G M Weinstock J Weissenbach S M Williams WoodageT K C Worley D Wu S Yang Q A Yao J Ye R F Yeh J S Zaveri M Zhan G Zhang Q Zhao L Zheng X H Zheng F N Zhong W Zhong X Zhou S Zhu X Zhu H O Smith R A Gibbs E W Myers G M Rubin J C Venter

The fly Drosophila melanogaster is one of the most intensively studied organisms in biology and serves as a model system for the investigation of many developmental and cellular processes common to higher eukaryotes, including humans. We have determined the nucleotide sequence of nearly all of the approximately 120-megabase euchromatic portion of the Drosophila genome using a whole-genome shotg...

2010
Manfred Claassen

Proteomics is a branch in biology that aims to comprehensively characterize a proteome. Mass spectrometry based proteomics has proven to be the most powerful approach to achieve this goal. This thesis introduces statistical concepts to optimally design and validate shotgun proteomics experiments and thereby enables to efficiently achieve reliable and extensive proteome coverage. The first part ...

2008
Marrett Grund

Lead (Pb) is a toxic metal and is a primary material in most bullets used to hunt white-tailed deer. We conducted a study to examine bullet fragmentation patterns and to assess lead levels in deer and domestic sheep using different types of bullets and firearms. The firearms we tested included a centerfire rifle, a shotgun, and an inline muzzleloader. For the centerfire rifle, we used lead bull...

Journal: :Genetics and molecular research : GMR 2007
M E Cantão J E Ferreira E G M Lemos

In DNA microarray experiments, the gene fragments that are spotted on the slides are usually obtained by the synthesis of specific oligonucleotides that are able to amplify genes through PCR. Shotgun library sequences are an alternative to synthesis of primers for the study of each gene in the genome. The possibility of putting thousands of gene sequences into a single slide allows the use of s...

Journal: :European Journal of Dentistry 2008

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