Shigella infection has been occurring in both epidemic and sporadic forms on Andaman and Nicobar Islands in India (92 to 94°E longitude, 6 to 14°N latitude; Bay of Bengal). Although acute gastrointestinal infection clinically resembling shigellosis has been reported on the islands frequently, bacteriological surveillance was only started in 1994. The surveillance data showed that shigellosis is...

The sequences of the first 50 residues of the tryptophan synthetase o( chains of Shigella dysenteriae, Salmonella typhimurium, and Aerobacter aerogenes were determined and compared with the corresponding sequences of Escherichia coli and Pseudomonas putida. On the basis of observed amino acid residue differences, and inferred nucleotide differences in the respective genes, similarity to the E. ...

Background: Recombinant vaccine technology is one of the most developed means in controlling infectious diseases. However, an effective vaccine against Shigella is still missing. Objective: To evaluate recombinant IpaC protein of Shigella as a vaccine candidate. Methods: In this study we cloned IpaC gene into an expression vector in prokaryotic system. The protein expression was evaluated by SD...

Mucosal epithelial cells in the intestine act as the first line of host defense against pathogens by increasing mucin production for clearance. Despite this fact, the underlying molecular mechanisms by which Shigella dysenteriae transduce mucin gene expression remain poorly defined. The goal of this study was to determine the role of Bone morphogenetic protein (BMP) pathway in mucin gene expres...

Shiga toxin-producing Escherichia coli serotype O117:K1:H7 is a cause of persistent diarrhea in travelers to tropical locations. Whole genome sequencing identified genetic mechanisms involved in the pathoadaptive phenotype. Sequencing also identified toxin and putative adherence genes flanked by sequences indicating horizontal gene transfer from Shigella dysenteriae and Salmonella spp., respect...

The failure of wastewater treatment plants to produce effluents of a high microbiological quality is a matter of great concern in terms of water resource pollution. A more serious concern is that this water source is used by communities in developing countries for multiple purposes, which include drinking, recreation and agriculture. The current study investigated the prevalence and potential h...

To assess the importance of TonB-dependent iron transport systems to growth of Shigella in vivo, a tonB mutant of Shigella dysenteriae was isolated and tested in cultured cells. The tonB mutant invaded epithelial cells, but did not form plaques in confluent monolayers of Henle cells, indicating an inability of this mutant to spread from cell to cell. The rate of intracellular multiplication of ...

BACKGROUND Shigella is a major cause of gastroenteritis especially in children. In developing countries, the incidence is frequent and results are often life threatening. Changing epidemiology and emerging antibiotic resistance warrants continuous monitoring of susceptibility. The present study highlights the changing epidemiology and drug resistance patterns of Shigella isolated at different h...

The aim of this study is to determine the antibacterial effects of different honey samples on clinically isolated bacteria species. Ginger (Zingiber officinales) and honey are one of the nature gifts to mankind and have been used to prevent and control disease conditions. The crude extracts of the plant materials were used with pure honey collected from various parts of Kogi State. The Agar dif...