Activated polymorphonuclear leucocytes (neutrophils) can generate both intracellular and extracellular luminol dependent chemiluminescence. As luminol dependent chemiluminescence largely measures the activity of the myeloperoxidase-H2O2 system, and as the extracellular activity of this enzyme may be responsible for the tissue damage associated with inflammatory conditions such as rheumatoid art...

The effect of 14 flavones on luminol-dependent chemiluminescence of neutrophils was studied in vitro. Chemiluminescence was used in this study as an indicator for the production of a reactive oxygen species by neutrophils, stimulated by phorbol myristate acetate. While flavone-8-acetic acid, and most of the compounds tested, inhibited chemiluminescence, flavone and its 5-hydroxy-7-methoxy deriv...

An investigation into the chemiluminescence of fourteen organic acids and tris(2,2'-bipyridyl)ruthenium(II) was undertaken. Particular emphasis was placed upon the method of production of the reagent, tris(2,2'-bipyridyl)ruthenium(III), with cerium(IV) sulfate, potassium permanganate, lead dioxide and electrochemical generation. Analytically useful chemiluminescence was observed when Ce(IV) or ...

A dual detection system for protein arrays is presented that combines label-free detection by optical interference with chemiluminescence. A planar protein array that targets hepatitis B surface antigen is developed. Surface densities for individual antibody spots are quantitated using optical interference prior to use. Target binding (10 ng/ml) is detected label-free. Target binding (1 ng/ml) ...

Rationale: Reactive oxygen species (ROS) and reactive nitrogen (RNS) are important regulators of inflammation. The exact impact ROS/RNS on cutaneous delayed-type hypersensitivity reaction (DTHR) is controversial. aim our study was to identify the dominant sources during acute chronic trinitrochlorobenzene (TNCB)-induced DTHR in mice with differently impaired production. Methods: TNCB-sensitized...

A new chemiluminescence method is proposed for the determination of sulfide in seawater based on the chemiluminescence reaction between sulfide and an acidic permanganate solution. 3-Cyclohexylaminopropanesulfonic acid was used as a chemiluminescence enhancer. By use of this method, 1-150 µM of sulfide could be determined in artificial seawater. The limit of detection was 0.17 µM sulfide. We in...

p-Iodophenol, p-coumaric acid, phenol and aniline are enhancers of the luminol-H202-horseradish peroxidase chemiluminescence. These enhanced and unenhanced chemiluminescences were used to assay low concentrations of hydrogen peroxide. The curves of cherniluminescence intensity maxima against hydrogen peroxide concentration were fitted to third order polynomial regressions, which permit a very g...

The principles of chemiluminescence and its applications as diagnostic tool are reviewed. After an introduction to the theoretical aspects of luminescence and energy transfer, the different classes of chemiluminogenic labels including luminol, acridinium compounds, coelenterazine and analogues, dioxetanes, systems based on peroxyoxalic acid and their derivatives are described emphasizing the mo...

A new chemiluminescence method using flow injection is described for the determination of four penicillins, namely: phenoxymethylpenicillin potassium, amoxicillin, ampicillin, and ampicillin sodium. The method is based on sensitizing effect of these drugs on the chemiluminescence reaction of potassium permanganate in sulfuric acid with glyoxal. The different experimental parameters affecting th...

Neutrophil chemiluminescence was determined in patients with active rheumatoid arthritis. Twelve patients were randomly assigned either to a diet high in polyunsaturated fatty acids supplemented with eicosapentaenoic and docosahexaenoic acids or to a diet high in saturated fatty acids. A correlation with clinical and laboratory parameters is also reported. No statistical difference was observed...