نتایج جستجو برای: semiquantitative rt pcr

تعداد نتایج: 201422  

Journal: :BioTechniques 1996
R J Klebe G M Grant A M Grant M A Garcia T A Giambernardi G P Taylor

Reverse transcription-PCR (RT-PCR) has traditionally required time-consuming RNA extraction and purification. This report demonstrates that one can completely avoid the RNA extraction step in RT-PCR by basing the comparison of samples on cell number rather than micrograms of total RNA. A new method for lysing cells while preserving RNA is described. RT-PCR is carried out (i) by rapidly freezing...

Journal: :Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 2013
Richard J Whitley Charles A Boucher Bruno Lina Jonathan S Nguyen-Van-Tam Albert Osterhaus Martin Schutten Arnold S Monto

BACKGROUND Following emergence of naturally occurring oseltamivir-resistant influenza A(H1N1) viruses, a global observational investigation, the Influenza Resistance Information Study (IRIS; NCT00884117), was initiated in 2008 to study neuraminidase inhibitor (NAI) resistance and clinical outcome. METHODS Patients with influenza-like illness and/or positive rapid test results agreed to swabs ...

Journal: :BioTechniques 2013

Journal: :Nucleic Acids Research 1993

Journal: :iranian journal of veterinary medicine 2010
مهدی سلطانی mohades ghasemi mostafa sharif rohani iesa sharif poor seyed jalil zoriyeh zahra

outbreaks causing mass mortalities have been observed in the golden grey mullet (liza auratus) population in southern coastal area of the caspian sea since 2002. the clinical signs include neurological abnormalities, such as erratic swimming behavior, spiral swimming, belly-up at rest and over inflation of the swim bladder. three hundred and twelve samples of moribund fish with sizes that range...

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