Fertility of frozen-thawed bull sperm is reduced by cryopreservation. Freezing-thawing procedures can result in as much as a sevenfold fertility decrease. Sperm mortality and loss of motility do not fully explain the reduced fertility of cryopreserved semen; they may be partially explained by the loss of sperm surface proteins, which are necessary for fertilization. We have previously identifie...

This study evaluated the effects of semen extension and storage on forward progressive motility % (FPM%) in agouti semen. Three extenders were used; sterilized whole cow's milk (UHT Milk), unpasteurized (CW) and pasteurized coconut water (PCW), and diluted to 50, 100, 150, and 200 × 10(6) spermatozoa/ml. Experiment 1: 200 ejaculates were extended for liquid storage at 5(∘)C and evaluated every ...

Although dog was the first species to be used for Artificial Insemination (AI) and become pregnant by Spallanzani in 1776, very little work has been done on preservation of dog semen to exploit the full genetic potential of pedigree males. Frozen semen is now inseminated routinely in cattle, sheep and goats and much research has been carried out with regard to the optimum conditions for its use...

BACKGROUND The influence of an accompanying person (parent, guardian or nurse) on the ability of an adolescent (post-pubescent, <20 years of age) to produce a semen sample for cryopreservation, is undetermined, as is the potential for use of urine samples to retrieve sperm in those adolescents that are unable to produce a semen sample. METHODS The records from 1991-2000 inclusive were reviewe...

Aim An experiment was designed to evaluate the role of Vitamin E and glutathione in improving the seminal parameters during hypothermic storage of liquid semen at 4°C for 72 h. Materials and Methods Thirty-six semen ejaculates were collected by artificial vagina from 6 bucks (Beetal) during the normal reproduction season (September to November) at weekly interval. The samples were centrifuged...

Artificial insemination (AI) is becoming more common in canine reproduction, permitting the use of shipped semen, assistance for geriatric or subfertile males, coverage of dominant females, and advanced reproductive technology such as intrauterine deposition of semen. AI may be performed with fresh, chilled, or frozen semen. All instruments should be clean and free of any chemical contamination...

Background: Many studies are being conducted to improve the quality of bull sperm in storage conditions. It seems that increasing sperm membrane unsaturated fatty acids can enhance sperm quality. This study was aimed to investigate the effects of combination of omega- 3, 6, 9 fatty acids on characteristics of bovine chilled semen. Materials and Methods: Different levels of oil containing omega-...

the present study investigated the possibility of replacing salamon with modified shotor diluent (msd) and egg yolk (ey) with low density lipoprotein (ldl) for chilled storage of ram semen. good quality semen (>80% progressive forward motility (pfm) of sperm) from 3 fertile rams was collected using an artificial vagina and pooled for each experiment. low density lipoprotein was extracted from f...

Aims: Determine the motility of spermatozoa in fresh goat semen after being given turmeric extract.&#x0D; Study Design: Randomized Block Design.&#x0D; Place and Duration Study: Sample: Galang, Deli Serdang Regency, Indonesia, between December 2022 January 2023.&#x0D; Methodology: Giving extract to once a day. The treatment included adding 0ml, 25 ml, 50 75 100 ml extract. parameter observed was...

The current study was performed to test the of quality as well as the DNA integrity of preserved liquid and frozen buffalo spermatozoa due to addition of different concentrations of glutathione (0.50, 1.00, 2.00 and 3.00 mM). Liquid semen was preserved for 120 hour with or without addition of glutathione and examined for progressive motility, viability, plasma and acrosomal membranes integrity ...