There is an increasing requirement to breed durable resistances to woolly apple aphid (WAA) into apple cultivars. Genetically diverse apple plantings have been established in New Zealand with one aim to identify new sources of resistance to this pest, and also to allow the computation of parameters of genetic interest. Such computations are hindered by the uneven distribution of the pest in the...

Genomic DNA represents the total genome of an organism and is valuable for different molecular studies. The isolation of high quality DNA is a prerequisite for these molecular techniques. The seeds of apple (Malus domestica) contain high amount of polysaccharides, polyphenols and other secondary metabolites that can hamper DNA isolation, amplification, restriction digestion and subsequent molec...

using apple clonal rootstocks is necessary for improving crop efficiency in apple orchards. after graftingof apple cultivars on malling rootstocks, the extra parts of the rootstock was used as stem cuttings. apical,middle and basal parts of these shoots were used as cuttings. two propagation conditions, greenhouse andnursery and three concentrations of indole-3- butyric acid (iba) on rooting, c...

High-density SNP arrays for genome-wide assessment of allelic variation have made high resolution genetic characterization of crop germplasm feasible. A medium density array for apple, the IRSC 8K SNP array, has been successfully developed and used for screens of bi-parental populations. However, the number of robust and well-distributed markers contained on this array was not sufficient to per...

to determine the specificity and host range of wilsonomyces carpophilus isolates, some stone fruit seedlings (prunus armeniaca, prunus dulcis, prunus reuteri, p. vulgaris, p. domestica, p. persica, p. avium and p. cerasus), pome fruits (malus communis, pyrus communis, crataegus azarollus and crataegus monogyna) as well as ornamental plants (rosa persica, rosa sp. and r. damascena) were selected...

Sweetness is one of the main drivers of consumer preference, and thus is given high priority in apple breeding programmes. Due to the complexity of sweetness evaluation, soluble solid content (SSC) is commonly used as an estimation of this trait. Nevertheless, it has been demonstrated that SSC and sweet taste are poorly correlated. Though individual sugar content may vary greatly between and wi...

Apple production in temperate regions is continuously imperiled by spring frosts. Economic losses caused frost damage can be enormous, and the risk of such projected to rise due global climate change. Induction bloom delay has been suggested as a potential avoidance strategy. However, applicable practices toward this goal are largely lacking, primarily complexity mechanisms governing dormancy b...

this study was carried out to examine the effects of kaolin spray for reducing the adverse effects of high radiation and temperature, especially on fruit sunburn in apple. treatments were four concentration of kaolin (0, 2, 4, and 6%) and two important and commercial apple cultivars in fars province; ‘golden delicious’ and ‘starking delicious’. trees physiological parameters, fruit sunburn and ...

We have used new generation sequencing (NGS) technologies to identify single nucleotide polymorphism (SNP) markers from three European pear (Pyrus communis L.) cultivars and subsequently developed a subset of 1096 pear SNPs into high throughput markers by combining them with the set of 7692 apple SNPs on the IRSC apple Infinium® II 8K array. We then evaluated this apple and pear Infinium® II 9K...

Manual cultivar identification diagram is a new strategy for plant cultivar identification based on DNA markers, providing information to efficiently separate cultivars. We tested 25 pairs of apple EST-SSR primers for amplification of PCR products from loquat cultivars. These EST-SSR primers provided clear amplification products from the loquat cultivars, with a relatively high transferability ...