AIMS Different cultural conditions for forming and reverting protoplasts were systematically studied to establish a rapid and efficient protocol for Sclerotium rolfsii ATCC 201126. METHODS AND RESULTS Osmotic stabilizer, lytic enzymes and mycelial age were the main factors influencing protoplast yields. An optimized protocol involving 1-h hydrolysis of 45-h-old mycelium with Trichoderma harzi...

sclerotium cepivorum incited white rot is one of the destructive diseases of garlic inhamedan province. a total number of 175 bacillus strains were isolated from soil and fromgarlic rhizospheres. the antagonism of the bacterial isolates against sclerotium cepivorum wasevaluated using dual culture method. ten high level activity isolates which produced inhibitionzones were selected for further s...

BACKGROUND Weight-loss medicines, including crude drugs and herbal supplements disguised as diet-aid products, are readily obtainable and distributed widely, especially in Southeast Asia. Even if such products are unapproved or prescription-only medicines, consumers can purchase them through an agency or directly on the Internet. We evaluated the quality and safety of herbal products purchased ...

International Poultry Production — Volume 14 Number 1 Ergot toxicity or ergotism is caused by ingestion of cereal or grass grains infected by the fungus Claviceps purpurea. The toxic agents (alkaloids) are produced by the fungus Claviceps purprea which attacks the embryo of the developing kernel forming a sclerotium, a black tangle of vegetative fungal strands. The sclerotium may contain more t...