background: this study was performed to induce conversion of rh strain tachyzoites of toxoplasma gondii to bradyzoites by ph changing of the culture medium. methods: hela cell monolayers were infected at a 1:1 tachyzoite to cell ratio. four hours after infection, the culture medium was removed and replaced with culture medium and 5% fcs, adjusted to ph 8 with naoh. the culture was maintained ...