background: rh (rhesus) is a highly complex blood group system in man deeply rooted in transfusion medicine. isolation of rhd from cord blod, cloning and expression of recombinant rhd antigen in bacterial expression system was the aim of this study. methods: total rnas were extracted from cord blood (o + ).  the quality of rna was determined by electrophoresis. in or­der to obtain coding sequen...

background: luteinizing hormone (lh) was secreted by the stimulating cells of the testes and ovaries in the anterior pituitary gland. the application of this hormone is in the treatment of men and women with infertility and amenorrhea respectively. m aterials and methods: in the present study the alpha and beta subunits of human lh gene were cloned into the pegfp-n1 expression vector and produc...

background: tuberculosis (tb) is a major cause of death worldwide. finding an effective vaccine against tb is the best way to control it. several vaccines against this disease have been developed but none are completely protective. the aim of this study was to design and construct a cloning vector containing the mycobacterium tuberculosis (m. tuberculosis) heat shock protein x (hspx). methods: ...

cloning and sequencing of a lipase gene from an indigenous bacillus pumilus, strain f3, revealed an open-reading frame of 648 nucleotides predicted to encode a protein of 215 residues. sequence analysis showed that f3 lipase contained a signal peptide composed of 34 amino acids with an h domain of 18 residues. a tat-like motif was found in the signal peptide similar to some other bacillus pumil...

objective: toxoplasmosis, caused by an intracellular protozoan parasite, and the toxoplasma gondii, is widespread throughout the world. in recent years, significant progress has been made in the identification of vaccine candidates which could induce a protective response. gra7, an excretory 29 kda toxoplasma gondii a dense granular antigen released by infected host cells. in tachyzoite-infect...

We discuss the usefulness of quantum cloning and present examples of quantum computation tasks for which cloning offers an advantage which cannot be matched by any approach that does not resort to it. In these quantum computations, we need to distribute quantum information contained in states about which we have some partial information. To perform quantum computations, we use state-dependent p...

Viruses are factors that can fluctuate insect populations, including honey bees. Most bee infecting viruses single positive-stranded RNA may not specifically infect bees and be hazardous to other pollinator insects. In addition, these could synergize with stressors worsen the population decline. To identify underlying detailed mechanisms, reversed genetic studies infectious cDNA clones of neces...

Object-oriented programmers often need to clone objects. Mainstream languages, such as C# and Java, typically default to shallow cloning, which copies just one object and aliases references from that object. Other languages, such as Eiffel, provide deep cloning. A deep clone is a copy of the entire object graph reachable from the cloned object, which could include many unnecessary objects. Alte...

An improved immune cloning algorithm based on clustering competition is proposed, which ensures the diversity of antibody population, effectively improves convergence speed and avoids immature convergence. The applied to packing problem achieves good results.

............................................................................................................................. 3 INTRODUCTION.................................................................................................................... 3 AIM..........................................................................................................................................