There is a clear clinical need for a bioactive bone graft substitute. Poly(vinyl phosphonic acid-co-acrylic acid) (PVPA-co-AA) has been identified as a promising candidate for bone regeneration but there is little evidence to show its direct osteogenic effect on progenitor or mature cells. In this study mature osteoblast-like cells (SaOS-2) and human bone marrow-derived mesenchymal stem cells (...

We know that the Yin Yang 1 protein (YY1) overexpression is positively and strongly correlated with the degree of malignancy of bone tumors. Therefore, we questioned whether we could influence cell invasiveness by deleting YY1 in human osteosarcoma cells (SaOs-2), as tested in Matrigel-coated filters and metastasis implantation of such osteosarcoma cells in vivo, by serial analysis with nuclear...

Protein phosphorylation and dephosphorylation has been recognized as an essential mechanism in the regulation of cellular metabolism and function in various tissues. Serine and threonine protein phosphatases (PP) are divided into four categories: PP1, PP2A, PP2B, and PP2C. At least four isoforms of PP1 catalytic subunit in rat, PP1α, PP1γ1, PP1γ2, and PP1δ, were isolated. In the present study, ...

objective: osteon has been introduced as a bone substitute material. it is biphasic calcium phosphate (bcp) that use in dentistry. the aim of the present study was to evaluate the effect of osteon on the proliferation, cell viability and differentiation of saos-2 cells in vitro. also it was compared with cerasorb bone graft. materials and methods: two different bone grafts materials, osteon an...

Purpose: Although osteosarcoma is the most common primary malignant bone tumor among adolescents and young adults, molecular mechanisms underlying development of cancer are not fully elucidated. Cyclophosphamide (CYC) an alkylating antineoplastic agent widely used in treatment regimens various cancers chronic inflammatory diseases. The aim this study to reveal effect CYC on Saos-2 cells at leve...

A háromdimenziós (3D) nyomtatást, dinamikusan fejlődő technikájának köszönhetően, az ipari területek után orvostudomány is egyre szélesebb körben alkalmazza. fogászatban elterjedt 3D nyomtatáshoz használt polimerek orális környezetben való használata során kulcskérdés a biokompatibilitás. Célkitűzésünk MED610 (Stratasys, USA) polimerből készített mintákból kioldódó komponensek azonosítása, vala...

Torsion gelometry, a fundamental rheological test in which specimens are twisted until they fracture, was applied to several different cheese varieties to determine its suitability for measuring their textural properties. Fresh and aged Brick, Cheddar, Colby, Gouda, Havarti, Mozzarella, and Romano cheeses were subjected to torsion analysis, and the results were compared with those from small am...

In this paper, the surface modification of titanium alloys Ti-15Mo, Ti-13Nb-13Zr, and Ti-6Al-7Nb is presented as a material for dental implants. The conditions of the plasma electrolytic oxidation process and alkali treatment were designed in this way to enhance the biological properties of the surface of promising Ti alloys. The differences in their surface morphology and, consequently, in the...

BACKGROUND Osteosarcomas are malignant bone tumors consisting of cells with abnormal cellular functions. Although osteosarcoma-derived cells are commonly used for osteoblastic models, the molecular composition of the osteosarcoma extracellular matrix (ECM) is not well characterized. MATERIALS AND METHODS We compared three osteosarcoma cell lines (MG-63, Saos-2 and U-2 OS) with normal human os...

Autophagy has been implicated in the progression and chemoresistance of various cancers. In this study, we have shown that osteosarcoma Saos-2 cells lacking ATG4B, a cysteine proteinase that activates LC3B, are defective in autophagy and fail to form tumors in mouse models. By combining in silico docking with in vitro and cell-based assays, we identified small compounds that suppressed starvati...