a total of 400 bovine diarrhoeic fecal specimens were obtained and conventional microbial culture, immunomagnetic separation and multiplex pcr were simultaneously carried out on samples. for detection of salmonella at genus level, inv-a universal primer was selected. in order to identifiing of salmonella typhimurium, specific primers of rfbj, fljb and flic related to gene sequence of o4, h2:1,2...