Serial Analysis of Gene Expression (SAGE) is a technology for measuring quantitatively the expression levels of all genes in a cell population simultaneously. In this thesis, I study what we could not learn, what we could learn, and how to learn what we could learn, from SAGE data. The first chapter of this thesis is a short introduction of the SAGE technology and another popular technology for...

Sage provides a scaled world in which real and simulated intelligence analysts work to solve realistic scenarios in innovative task environments. All aspects of Sage are instrumented for data collection and Sage itself is built to facilitate the swapping in and out of prototypes of innovative tools for information search, hypothesis generation, and hypothesis testing. Sage focuses not simply on...

Organophosphate resistant and susceptible tick larvae from laboratory strains of the southern cattle tick, Rhipicephalus (Boophilus) microplus were exposed to low doses of the organophosphate (OP) acaricide, coumaphos. Serial analysis of gene expression (SAGE) was used to analyse differential gene expression in response to OP treatment and to compare the responses of OP-treated and -untreated r...

Serial analysis of gene expression (SAGE) technology produces large sets of interesting genes that are difficult to analyze directly. Bioinformatics tools are needed to interpret the functional information in these gene sets. We present an interactive web-based tool, called Gene Class, which allows functional annotation of SAGE data using the Gene Ontology (GO) database. This tool performs sear...

Salvia officinalis (common sage) is a plant with antidiabetic properties. A pilot trial (non-randomized crossover trial) with six healthy female volunteers (aged 40-50) was designed to evaluate the beneficial properties of sage tea consumption on blood glucose regulation, lipid profile and transaminase activity in humans. Effects of sage consumption on erythrocytes' SOD and CAT activities and o...

The serial analysis of gene expression (SAGE) is a powerful method to compare gene expression of mRNA populations. To provide quantitative expression levels on a genome-wide scale, the Cancer Genome Anatomy Project (CGAP) uses SAGE. Over 7 million SAGE tags, from 171 human cell types have been assembled. The growing number of laboratories involved in SAGE research necessitates the use of softwa...

In this study, we evaluate the biosafety and bioactivity (antioxidant potential) of a traditional water infusion (tea) of common sage (Salvia officinalis L.) in vivo in mice and rats by quantification of plasma transaminase activities and liver glutathione-S-transferase (GST) and glutathione reductase (GR) enzyme activities. The replacement of water by sage tea for 14 days in the diet of rodent...

PURPOSE To identify the genes expressed in normal human trabecular meshwork tissue, a tissue critical to the pathogenesis of glaucoma. METHODS Total RNA was extracted from human trabecular meshwork (HTM) harvested from 3 different donors. Extracted RNA was used to synthesize individual SAGE (serial analysis of gene expression) libraries using the I-SAGE Long kit from Invitrogen. Libraries wer...

Sage (Salvia officinalis L.) is used as an herbal medicinal product, with the most typical form of application as infusion with boiling water (sage tea). The well-established traditional uses include symptomatic treatment of mild dyspeptic complaints, the treatment of inflammations in the mouth and the throat, and relief of excessive sweating and relief of minor skin inflammations. In this stud...

The sage grouse (Crentrocercus urophasianus) is the largest North American grouse and depends on sagebrush (Artemisia spp.) for diet and protective cover. The association with sagebrush limits the range of sage grouse to the range of sagebrush. As a result of the loss of native sagebrush-grasslands, the range of sage grouse has decreased by over 50% since the turn of the century. Sage grouse ar...