Monovalent Fab fragments from immunoglobulins, directed against Escherichia coli small ribosomal subunit proteins, were tested for their effect on poly(U)-directed synthesis of polyphenylalanine by rat liver ribosomes. Of the 18 Fabs tested, 3, anti-S10, anti-S12, and anti-S14, inhibited polyphenylalanine synthesis; antibodies against 3 of the 21 E. coli ribosomal proteins (S1, S16, and S17) we...

Assembly of 30S ribosomal subunits from their protein and RNA components requires extensive refolding of the 16S rRNA and is assisted by 10-20 assembly factors in bacteria. We probed the structures of 30S assembly intermediates in E. coli cells, using a synchrotron X-ray beam to generate hydroxyl radical in the cytoplasm. Widespread differences between mature and pre-30S complexes in the absenc...

TABLE OF CONTENTS I. Preface .................................................................................................................................................................................S1 II. Glossary...............................................................................................................................................................................S...

Initiation factor-3 has been photochemically cross-linked to Escherichia coli 30 S ribosomal subunits by means of near-ultraviolet (> 285 nm) irradiation. The cross-linking was judged to be specific since noncovalent binding was required for subsequent cross-linking and was prevented by the presence of 0.5 M NH4Cl or 0.5 mM aurintricarboxylic acid. Covalent attachment reached its maximum level ...

Müller cells are the main glial cells in the retina, and are related to plexiform layer activity. Recent studies have demonstrated that Müller cells are involved in the synaptic conservation, plasticity, development and metabolism of glutamate. During turtle retinal development, layers, cells and synapses appear at different times. The aim of this research is to study the emergence of Müller ce...

The genomes of several species of mycoplasma have been sequenced. Most of these species rely on the glycolytic pathway for energy production, with the one exception of Ureaplasma, a species that breaks down urea as its principle source of acquiring energy. Several species, including as Mycoplasma arthritidis, are nonglycolytic and can use arginine as their source of energy. Described here are t...

The innate reactivity of the peptide melittin (H-GIGAVLKVLTTGLPALISWIKRKRQQ-NH(2)) towards membrane lipids has been explored using LC-MS methods. The high sensitivity afforded by LC-MS analysis enabled acyl transfer to the peptide to be detected, within 4 h, from membranes composed of phosphocholines (PCs). Acyl transfer from PCs was also observed from mixtures of PC with phosphoserine (PS) or ...

Introduction S1 Task Force on Overview of Training in Pediatric Gastroenterology and Nutrition S2 Task Force on Training in Gastrointestinal Motility and Functional Disorders S6 Task Force on Training in Acid–Peptic Disease S8 Task Force on Training in Short-Bowel Syndrome and Congenital Abnormalities of the Gastrointestinal Tract S10 Task Force on Training in Inflammatory Bowel Diseases S11 Ta...