the major aim of this study was to compare the morphology and activity of rumen protozoa of khuzestan water buffalo and holstein cow using in vitro digestibility and gas production parameters of steam treated sugarcane pith. rumen fluid obtained from two buffalo and cow steers fed the same diet, 30:70 concentrate: forage. to separate rumen protozoa, antibiotic solution and fungicides were added...

rumen is a persistent and specific ecosystem consists of bacteria, protozoa and fungus where feed fermentation takes place in it. produced hydrogen in rumen can be used in the synthesis of the volatile fatty acids and the microbial protein and its excess would be eliminated through the production of methane by methanogenesis. nutritionists have tried to find ways to decrease loss and energy and...

Degradation of plant N in the rumen results in the formation of ammonia-N and NAN. Rumen bacteria utilise these breakdown products to synthesise their cell proteins. This study was carried out to investigate the in vivo degradation of N-labelled duckweed in the rumen and the utilisation of the degradation products by rumen bacteria. We found that duckweed protein was relatively resistant to rum...

Rumen nitrogen metabolism values were estimated by the use of a single injection of 15(NH4)2SO4 into the rumen of sheep and consecutive 15N enrichment measurements in the rumen ammonia pool, rumen non-NH3-N (NAN) pool, rumen purine pool and blood urea-N (BUN) pool for a period of 24 h. Synthesis and degradation of N compounds in the rumen and passage of N to and from the rumen were evaluated on...

Dietary carbohydrates can be divided into two basic fractions: fibre and nonfibre carbohydrates (NFC). Fibre in dairy cow rations is essential for animal health, since it is required to support an appropriate rumen function and physiology. Therefore, ruminants require fibre in coarse physical form for a more effective chewing and ruminal activity. Increasing fibre content and forage particle si...

Amplicon sequencing utilizing next-generation platforms has significantly transformed how research is conducted, specifically microbial ecology. However, primer and sequencing platform biases can confound or change the way scientists interpret these data. The Pacific Biosciences RSII instrument may also preferentially load smaller fragments, which may also be a function of PCR product exhaustio...

High concentrations of the ciliate Epidinium Crawley are associated with damaged regions of fresh plant material undergoing digestion in the sheep rumen. This finding supports that postulate that sequestration in the rumen explains the low rate of passage of protozoa despite the high flow rate of liquid from the rumen. The maintenance of Epidinium in the rumen, despite their slow growth rate, i...

Local feed resources, particularly low-quality roughages and agricultural crop-residues, are of prime importance for ruminants raised in the tropics. These feeds exhibit close relationships with rumen ecology, microbes and rumen fermentation patterns. A number of dietary factors could influence rumen fermentation especially the basal roughage source, its physical form and fermentation end-produ...

This study was conducted to investigate the effect of feed types on Ochratoxin A (OTA) degradation by Korean native goats. Rumen fluid from canulated goats fed whole roughage or 50% roughage served as a source of micro-organisms. Experiments were undertaken i) to investigate OTA degradation ability in a 2×4 factorial arrangement with different feed types (100% roughage vs. 50% roughage) and rum...

The activities of phosphoenolpyruvate carboxykinase, ;malic enzyme', citrate-cleavage enzyme and glucose 6-phosphate dehydrogenase were assayed in homogenates of rumen mucosa, liver and adipose tissue of cattle. Rumen mucosa cytoplasm contained activities of ;malic enzyme' approximately sevenfold those of phosphoenolpyruvate carboxykinase, suggesting that the conversion of propionate into lacta...