As a matter of fact, infectious diseases hamper everyone’s life and produce lifelong threat to everyone neutral age, sex, lifestyle socio-economic status. Nowadays, come into the sight Chikungunya viral (CHIKV) infection injured many Asian African countries, also deliberated in rising countries low countries. CHIKV is positive-sense, enveloped single-stranded, RNA virus belonging genus Alphavir...

We developed a loop-mediated isothermal amplification (LAMP) method to rapidly diagnose Wheat streak mosaic virus (WSMV) during quarantine inspections of imported wheat, corn, oats, and millet. The LAMP method was developed as a plant quarantine inspection method for the first time, and its simplicity, quickness, specificity and sensitivity were verified compared to current reverse transcriptio...

An artificial intelligence-assisted low-cost portable device for the rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is presented here. This standalone temperature-controlled houses tubes designed conducting reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays. Moreover, utilises illuminated by LEDs, an in-built camera, and a small onboar...

Nucleic acid amplification is the basis for many molecular diagnostic assays. In these cases, the amplification product must be detected and analyzed, typically requiring extended workflow time, sophisticated equipment, or both. Here we present a novel method of amplification detection that harnesses the pH change resulting from amplification reactions performed with minimal buffering capacity....

Because of the serological cross-reactivity among the flaviviruses, molecular detection methods, such as reverse-transcription polymerase chain reaction (RT-PCR), play an important role in the recent Zika outbreak. However, due to the limited sensitivity, the detection window of RT-PCR for Zika viremia is only about one week after symptom onset. By combining loop-mediated isothermal amplificati...