We examined effects of mist quantity, topophysis (origin of cutting in the crown of 6-year-old trees), maturation state of the donor stock, and time of sticking on rooting and root system quality of cuttings representing five full-sib hybrid larch families obtained with Larix decidua Mill., L. laricina (du Roi) K. Koch, and L. kaempferi (Lamb.) Sarg. (Sieb. and Zucc.) Gord., as parents. Mist fr...

Pulmonary ventilation and pulmonary arterial pressure both rise progressively during the first few hours of human acclimatization to hypoxia. These responses are highly variable between individuals, but the origin of this variability is unknown. Here, we sought to determine whether the variabilities between different measures of response to sustained hypoxia were related, which would suggest a ...

In this study, silver nanoparticles effects on the sterilization of different sources explants (young leaves, young flower stalks and buds) Gerbera as well in vitro morphogenesis their growth were investigated. The sterilized cut transversally (1 mm) with stalk, square (0.5 × 0.5 cm) for leaf sample, longitudinally flowers (removed petals) cultured MS medium; then, (contamination-free or no bro...

Semi-hardwood cuttings of ‘Frantoio’ (high rooting ability) and ‘Gentile di Larino’ (low rooting ability) cultivars were obtained from 1-year-old olive shoots sampled in mid-August during the 2000 and 2001 growing season. Semi-hardwood cuttings were dipped in IBA 4000 ppm and H2O2 (0%-control or 3.5% w/v) solutions before rooting in greenhouse equipped with an automatic mist system. At 57 and 8...

We investigated the influence of cutting water potential (Psicut) on rooting of juvenile hardwood (dormant) and softwood (succulent) stem cuttings of loblolly pine (Pinus taeda L.) propagated under varying substrate water potentials (Psisub) and volumes of mist application. Mist treatment and Psisub contributed to the Psicut of unrooted stem cuttings. When Psisub was held constant across mist t...

The development of adventitious roots is affected by several factors, including the age of the cutting donor plant, which negatively affects rooting capacity. Eucalyptus globulus quickly loses rooting capacity of cuttings as the donor plant ages, although the molecular and biochemical mechanisms behind this process are still unclear. To better understand the bases of rooting competence loss in ...

A micropropagation protocol was developed which may assist in the safeguarding and augmentation of dwindling natural populations of Clinopodium odorum (Griseb.) Harley, a critically and endangered medicinal plant. Factors affecting culture initiation bud sprouting and growth, rooting, and acclimatization were studied, using nodal segments of in vitro germinated seedling as primary explants on s...

Apical meristems and nodal plant parts were used for mass propagation of Peperomia obtusifolia. Different concentrations of BAP (6-benzylaminopurine), TDZ (Thidiazuron) and KIN (Kinetin) were used in MS medium. The highest shoot proliferation and multiplication formation occurred in the MS basal medium containing 1.0 mg/mL BAP. Maximum number of shoots and shoot lengths were 9.80 ±0.50 and 35.4...

RAPD analysis This study aimed to develop an efficient method for micrpropagation of true-to-type date palm plants through direct organogenesis. Nodular cultures were obtained from shoot tips on MS medium plus 2 mg/l 2ip and 1 mg/l NAA. Among combinations of 2ip and 2,4-D added to culture medium for direct shoot buds proliferation, 5 mg/l 2ip alone gave the highest organogenesis frequency. For ...

Abstract In vitro culture techniques are recognized as efficient strategies for large-scale plant production, well providing alternatives conservation. this study the micropropagation of Tarenaya rosea was established using petiole and foliar blade segments cultivated on MS medium with 6-benzyladenine (BA) and/or 6-furfurylaminopurine (KIN). The regeneration rate from explants evaluated after 3...