Morphological, anatomical and physiological features of in vitro plantlets are affected by the environmental conditions experienced. The high water content inside in vitro-formed tissues makes difficult to maintain the original characteristics of samples from in vitro plantlets during the preparation procedures for morphological and anatomical studies. In this paper, micropropagated Vitis vinif...

Plant somatic cells have the remarkable ability to regenerate an entire organism. Many species in the genus Kalanchoë, known as "mother of thousands," develop plantlets on the leaf margins. Using key regulators of organogenesis (STM) and embryogenesis (LEC1 and FUS3) processes, we analyzed asexual reproduction in Kalanchoë leaves. Suppression of STM abolished the ability to make plantlets. Here...

ABSTRACT Gerbera (Gerbera jamesonii Bolus ex Hook. F.; Asteraceae), is one of the most economically important ornamental plants due to its aesthetic value. In present study, we established a micropropagation method for large-scale production quality planting material gerbera ‘Pink Melody’. Eighty-six percent capitulum explants produced adventitious shoots (15.44 ± 0.34 per capitulum) on Murashi...

Ochradenus baccatus is a medicinal plant of high value, spread over sandy and stony places of Kingdom of Saudi Arabia and most of the desert regions of Egypt. This species contains several flavanoids and specific constituents which are important as these have already been effectively used in lowering cholesterol in the blood of rats and high inhibition potential of the malarial parasite (Plasmo...

Background and aims Dendrobium hookerianum is a rare and threatened epiphytic orchid of northeast India. Prospects for conservation would be strengthened by developing an in vitro method for mass propagation. Seeds are minute and difficult to use directly in the field for this purpose, being non-endospermous with a low nutrient content and dependent on a specific fungus for germination and earl...

As interest in how to increase biomass production through biotechnological means gains traction, focus is turning towards the use of photoautotrophic micropropagation under elevated levels of carbon dioxide (CO2) to maximize plant growth and productivity. The effect of super-elevated CO2 with cold cathode fluorescent lamps (CCFLs) on the photoautotrophic growth of Oncidesa in vitro has been stu...

An autotrophic culture system was developed for the in vitro mycorrhization of potato plantlets. Roots of micropropagated plantlets were associated to an arbuscular mycorrhizal fungus under in vitro conditions, while shoots developed under open air conditions. Several thousand spores, an extensive extraradical mycelium and an abundant root colonization were obtained. Spores were able to coloniz...

Date palm tissue culture technique aimed to production of huge numbers of plants to farmers in order to increasing dates which have high nutritional value. Date palm plantlets from pre-acclimatization stage in vitro were transferred to acclimatize in the greenhouse cultured in peat moss + perlite 2:1 and kept in the plastic covered inside 100% humidity and 28 C0 till new leave or root was grown...

Plantlets under in vitro conditions transferred to ex vivo conditions are exposed to biotic and abiotic stresses. Furthermore, in vitro regenerated plants are typically frail and sometimes difficult to handle subsequently increasing their risk to damage and disease; hence acclimatization of these plantlets is the most important step in tissue culture techniques. An experiment was conducted unde...