We demonstrate that the topoisomerase activity of bacteriophage lambda Int protein introduces single-strand breaks into duplex DNA at specific sites. Strand breakage is accompanied by the covalent linkage of Int to DNA. The linkage connects a residue in Int to the 3' phosphate of DNA at the site of breakage; the other breakage product has a 5' OH terminus. Int is the first procaryotic topoisome...

this study aimed to investigate effects of malathion on dna breakage in the liver and gill of rainbow trout (oncorhynchus mykiss) in the laboratory condition. the experiment was performed with four concentrations of malathion, i.e. 0 (control), 0.025, 0.05 and 0.075 mg l‑1 and three replicate for nine days. the liver and gill were sampled on the first, fifth and ninth days after the start of th...

Detection of tool breakage is very important for automated machining operations. This paper presents a negative-selection algorithm for tool breakage detection. The method is inspired by the defense mechanism of the immune system, which discriminates between self and non-self. Here self is deened to be normal cutting operations and non-self is any deviation beyond allowable variation of the cut...

A novel multicolor fluorescence in situ hybridization approach, using an alpha satellite probe which labels the centromeric region on chromosome 1 and a classical satellite probe which targets an adjacent breakage-prone region (1q12), has been used to detect both hyperdiploidy and chromosome breakage in interphase human cells. With the use of this technique significant increases in chromosomal ...

BACKGROUND Previous research reveals an association between traumatic stress and an increased risk for numerous diseases, including cancer. At the molecular level, stress may increase carcinogenesis via increased DNA damage and impaired DNA repair mechanisms. We assessed DNA breakage in peripheral blood mononuclear cells from individuals with post-traumatic stress disorder (PTSD) and measured t...

B susceptibility and hardness of three yellow dent corn hybrids were determined for kernels separated into 6 size and shape categories and equilibrated to 12.3 and 14.7% moisture (wet basis). Measurements for breakage susceptibility by severe impact from the Wisconsin Breakage Tester (WBT) and by a grinding action from the Stein Breakage Tester (SBT) were determined by sieve and by a green dye ...

background: this study has used biomarker of exposure-effect continuum to examine the biological characteristics of organophosphate (op) toxicity and its genotoxic effect among rice farmers.   materials and methods : a cross-sectional study was conducted among 160 pesticide exposed rice farmers and 160 adults from the fishing village as the unexposed group. they share the common socio-economica...

Using DEM (Discrete Element Method), a model is presented to simulate the breakage of two-dimensional polygonshaped particles. In this model, shapes of the particles generated after breakage are predefined and each uniform (uncracked) particle is replaced with smaller inter-connected sub-particles. If the bond between these sub-particles breaks, breakage will happen. With the help of this model...

Mobile Ad hoc Network (MANET) consists of a group of mobile nodes that can communicate with each other without the need of infrastructure. The movement of nodes in MANET is random; therefore MANETs have a dynamic topology. Because of this dynamic topology, the link breakages in these networks are something common. This problem causes high data loss and delay. In order to decrease these problems...

In recent years, heat treatment has attracted attention as a means to improve the color sorting technology with the aim of improving the quality of low-grade limestone. The crucial stage in this technique is to evaluate the breakage characteristics of the sample materials after color sorting. In this study, the breakage characteristics of samples showing color differences after heat treatment a...