background: glucose-6-phosphate dehydrogenase (g6pd) deficiency is the most common disease of the hexose monophosphate pathway existing in more than 400 million people worldwide. the aim of this study was to identify neonates with g6pd deficiency following national program for screening and education of affected newborns’ parents started since june 2007 in mazandaran, a northern province of ira...

background and objectives: proteases are a group of enzymes that catalyze the degradation of proteins resulting in the pro- duction of their amino acid constituents. they are the most important group of industrial enzymes which account for about 60% of total enzymes in the market and produced mainly by microorganisms. the attempts were made to study the kinetic parameters of protease produced b...

objective(s):diabetes plays an important role in the induction of the liver injury. grape seed proanthocyanidin (gsp) have a wide range of medicinal properties against oxidative stress. in this study we evaluated antioxidant effects of gsp on liver in streptozotocin-induced diabetic rats. materials and methods: thirty male sprague–dawley rats were divided into three groups: control, untreated d...

a pancreatopeptidase (elastase) digest index was devised for a rapid and accurate estimation of protein quality. this index was calculated on the basis of all the amino acids released by an in-vitro elastase digestion, acid hydrolyses of same sample and the residue of enzyme hydrolyzed. the amino acids were determined by thin-layer chromatography. samples used were cooked white kidneybeans, coo...

abstract cyanide is a colorless, harmful, and dangerous substance with a smell similar to bitter almond. cyanide forms a stable compound with iron and magnesium and interferes with cell activity by interfering with respiration, carbon stabilization and nitrate revival. in order to evaluate cyanide metabolism in fuji and abassi mashhad apples, a factorial experiment was conducted as split plot b...

abstract background: hereditary red cell enzyme disorders are a group of non-immune/spherocytic hemolytic anemia, although these disorders are rare and they have not public health problems, the detection of these defects could help to physician in treatment and differential diagnosis. this study evaluated 5 enzymopathies in patients with hereditary non –immune/spherocytic hemolytic anemia (hnsh...

Glucose 6-phosphate dehydrogenase (G6PD) was purified from Streptomyces aureofaciens and inactivated with butanedione and diethylpyrocarbonate. Incubation of the enzyme with butanedione resulted in a rapid activity loss (80%) within 5 min, followed by a slow phase using a molar ratio to enzyme concentration of 100. Fluorescence studies showed a conformational change in the butanedione-modified ...