OBJECTIVES Studies which consider the molecular mechanisms of degeneration and regeneration of cartilaginous tissues are seriously hampered by problematic ribonucleic acid (RNA) isolations due to low cell density and the dense, proteoglycan-rich extracellular matrix of cartilage. Proteoglycans tend to co-purify with RNA, they can absorb the full spectrum of UV light and they are potent inhibito...

BACKGROUND Gene expression profiling is a useful tool for cancer diagnosis and basic research. A major limitation is that, even during short-term storage of native specimens of peripheral blood or bone marrow (BM) and/or RNA isolation, significant changes of gene expression pattern can occur because of gene induction, repression, and RNA degradation. METHODS We investigated the effectiveness ...

SUPPLEMENTAL MATERIALS & METHODS: TABLE OF CONTENTS I. PRIMARY DATA GENERATION .........................................................................................3 Cell Culture and Cell Lines Assayed .....................................................................................................................................3 Sub-cellular Fractionation and RNA Isolation (CSHL)......

One major problem associated with collecting whole blood from patients for use as a source of RNA in gene expression studies is that the RNA degrades during collection and storage. Preservation of RNA quality is vital in such studies because the stability of the RNA ultimately affects analysis of gene expression. In this study the PAXgene blood collection system was compared with a standard ery...

RNA isolation is performed everyday by molecular biologists for a wide range of applications. No matter what protocol is used and how meticulous the user is, a risk to degrade RNA during the process or to carry over contaminants always exists which may affect the reliability of the subsequent analytical techniques. The parameters that are usually checked for quality control include the concentr...