We created a panel of 10 representative multi-nonnucleoside reverse transcriptase inhibitor (NNRTI)-resistant recombinant infectious molecular HIV-1 clones to assist researchers studying NNRTI resistance or developing novel NNRTIs. The cloned viruses contain most of the major NNRTI resistance mutations and most of the significantly associated mutation pairs that we identified in two network ana...

Laurent Mandelbrot, Dominique Duro, Emilie Belissa, Gilles Peytavin Assistance Publique-Hôpitaux de Paris, Hôpital Louis Mourier, Service de Gynécologie-Obstétrique, Hôpitaux Universitaires Paris-Nord Val de Seine, Colombes, France; Université Paris-Diderot, Université Sorbonne Paris-Cité, DHU Risks in Pregnancy, Paris, France; INSERM U1018, Kremlin-Bicêtre, France; Assistance Publique-Hôpitaux...

OBJECTIVE To determine drug resistance mutation (DRM) patterns in a large cohort of patients failing nonnucleoside reverse transcriptase inhibitor (NNRTI)-based first-line antiretroviral therapy regimens in programs without routine viral load (VL) monitoring and to examine intersubtype differences in DRMs. DESIGN Sequences from 787 adults/adolescents who failed an NNRTI-based first-line regim...

Incidence of brain infections in Human Immunodeficiency Virus (HIV) positive patients is reduced after the availability of current high active antiretroviral therapy (HAART). Herpes Simplex Virus type 2 (HSV-2) is an infrequent cause of encephalitis in HIV patients despite it is frequently involved in sexual transmitted infections. Here, we report a case of HSV-2 encephalitis occurring in a pat...

OBJECTIVES The aim of this study was to prospectively survey transmitted drug resistance (TDR) among recently infected individuals (mostly MSM). METHODS TDR was determined in prospective annual cohorts of recently HIV-1-infected individuals consecutively recruited from 2008 to 2010. Resistance interpretation was carried out using Stanford Database tools and the WHO surveillance drug resistanc...

4 5 6 7 Julieta Díaz-Delfín , Pere Domingo , Ma Gracia Mateo, Ma el Mar Gutierrez, Joan Carles 8 Domingo, Marta Giralt , Francesc Villarroya 9 10 11 Department of Biochemistry and Molecular Biology and Institut de Biomedicina (IBUB), University 12 of Barcelona and CIBER Fisiopatología de la Obesidad y Nutrición, and Infectious Diseases Unit, 13 Hospital de la Santa Creu i Sant Pau, Autonomous U...

538. A Retrospective Study to Evaluate the Safety of Switching Antiretroviral Therapy (ART) to Rilpivirine/Tenofovir Disoproxil Fumarate/Emtricitabine (RPV/TDF/FTC) STR in Virologically Suppressed HIV-Infected Patients Ana Monczor, MD; Xiaoying Yu, MD, MS; Karen J. Vigil, MD; Claudia Kozinetz, PhD, MPH; Roberto Arduino, MD; FUNCEI Fundacion Centro de Estudios Infectologicos, Buenos Aires, Argen...

Clustering of 99 available X-ray crystal structures of HIV-1 reverse transcriptase (RT) at the flexible non-nucleoside inhibitor binding pocket (NNIBP) provides information about features of the conformational landscape for binding non-nucleoside inhibitors (NNRTIs), including effects of mutation and crystal forms. The ensemble of NNIBP conformations is separated into eight discrete clusters ba...