The diagnostic value of the low-molecular mass proteins ribonuclease, beta 2-microglobulin, and lysozyme in serum for the detection of reduced glomerular filtration rates was evaluated. The values of these proteins and of serum creatinine investigated in 52 patients suffering from chronic renal diseases were plotted against 99m-Tc-diethylenetriaminopentaacetate clearance as an indicator of glom...

Digestion of yeast nucleic acid by ribonuclease is accompanied by a shift in the ultraviolet absorption spectrum of the substrate towards the shorter wave-lengths (Fig. 1). The shift is not due to the liberation of free acid which takes place during digestion (1, 2), since the effect of increasing the hydrogen ion concentration is to shift the absorption spectrum of yeast nucleic acid in a dire...

The RNA cleavage activity of RNase P can be employed to decrease the levels of specific RNAs and to study their function or even to eradicate pathogens. Two different technologies have been developed to use RNase P as a tool for RNA knockdown. In one of these, an external guide sequence, which mimics a tRNA precursor, a well-known natural RNase P substrate, is used to target an RNA molecule for...

'Thie nor-mal course of mitosis in plaint anid aniimal cells cali be disrutpted hy miianiy kinids of physical anid chemical agents. Nuclear tranisfoirmations esseiltial to the orderly progress of mitosis can be retarded, chromosome structure deformed, spilndle mechanisms impaired, and cytokinietic processes inhibited. Such disturbaiiees may be revealed, on cytological examinationi of the treate...

The aim of this review is to draw a correlation between the structure of the DNA/RNA hybrid and its properties as a substrate for the RNase H, as well as to point the crucial structural requirements for the modified AONs to preserve their RNase H potency. The review is divided into the following parts: (1) mechanistic considerations, (2) target RNA folding-AON folding-RNase H assistance in AON/...

Previous communications have reported on the reduction of the disulfide bonds of ribonuclease (14)) ribonuclease-S-protein (5), and lysozyme (6) by treatment of the native protein or protein derivative in 8 M urea solutions with thioglycolic acid or mercaptoethanol. Reduction by this procedure has a significant advantage over certain other techniques’ (e.g. borohydride (7) or sulfite reduction ...

Many studies on ribonuclease would benefit if reproducible, purified samples of the enzyme were readily available. The present report summarizes experiments on the preparation and storage of chromatographically purified bovine pancreatic ribonuclease A.’ Much of the earlier work on ribonuclease was done with a single, large, commercial batch of material (Armour Lot 381059) which was isolated fr...

The effects of endotoxin shock, endotoxin tolerance, and lead acetate plus a minute amount of endotoxin on the serum ribonuclease activity of rats was measured. Changes in serum ribonuclease activity after various entoxin treatments could be a primary effect or a secondary effect of the damaging effect of endotoxin.

The reaction of iodoacetic acid with ribonuclease has been studied recently by Gundlach et al. (1). Inactivation of the enzyme occurred over a wide range of pH. The authors concluded that at acid pH the reaction involved solely methionyl residues, that at pH 5.5 a histidyl residue was the principal site of attack, and that at alkaline pH values significant reaction at lysyl residues occurred. O...

A nitrile-labeled amino acid, p-cyanophenylalanine, is introduced near the active site of the semisynthetic enzyme ribonuclease S to serve as a probe of protein dynamics and fluctuations. Ribonuclease S is the limited proteolysis product of subtilisin acting on ribonuclease A, and consists of a small fragment including amino acids 1-20, the S-peptide, and a larger fragment including residues 21...