A sensitive and specific double antibody radioimmunoassay has been developed capable of measuring LH-RH in extracted human plasma. Thyrotropin releasing hormone, lysine vasopressin and most of LH-RH analogues did not appear to affect the assay. Hypothalamic extract and some of the LH-RH analogues produced displacement curves which were parallel to that obtained with the synthetic LH-RH. Sensiti...

Human erythroleukemic K562 cells are known to have several erythroid properties. K562 cells possess Rh mRNAs, but expression of Rh proteins has not previously been reported. We immunoprecipitated Rh protein from K562 cell lysate using rabbit anti-Rh and detected Rh(D) antigens on K562 cells using fluorescence-activated cell sorting (FACS). These results suggest that K562 cells will be useful as...

RH genes are highly polymorphic and encode the most complex of the 35 human blood group systems. This genetic diversity contributes to Rh alloimmunization in patients with sickle cell anemia (SCA) and is not avoided by serologic Rh-matched red cell transfusions. Standard serologic testing does not distinguish variant Rh antigens. Single nucleotide polymorphism (SNP)-based DNA arrays detect many...

Addition of Li, Na, K and Cs to a 1.5 wt% Rh/Al,O, catalyst resulted in a decrease of the hydrocarbon formation rate, while the methanol formation rate was almost unaffected. The overall result is an increased oxygenate selectivity at a decreased total activity. The oxo-selectivity increased in the order unpromoted < Li < Na< K < Cs. The results suggest that the main role of the alkali promoter...

Objective Anti-Rh D IgG is used for the prevention of anti-D antibody production in Rh- individuals who have been exposed to Rh+ red blood cells. The stability of IgG preparations as a solution is low, with a shelf life of a year or more. Formulation of anti-Rh D IgG as a lyophilized preparation would decrease its degradation rate and increases its shelf life. The objective of this study was t...

The RBC Rh antigens are of large clinical importance, but until recently have been poorly understood at a molecular level. The Rh polypeptides are a family of nonglycosylated Mr 30- to 32-Kd transmembrane proteins that are core structural components of the Rh antigens and have been purified and partially characterized biochemically. Rh polypeptides are present in RBCs from normal humans and oth...

The dihydrido germyl complex cis,fac-[Rh(GePh3)(H)2(PEt3)3] (2) was synthesized by an oxidative addition of HGePh3 at [Rh(H)(PEt3)3] (1). Treatment of 2 with neohexene generated the rhodium(i) germyl complex [Rh(GePh3)(PEt3)3] (3). Alternatively, treatment of the methyl complex [Rh(CH3)(PEt3)3] (4) with HGePh3 furnished at room temperature also 3. Low-temperature NMR measurements revealed an in...

Background One of the most important cause of severe neonatal hyperbilirubinemia is hemolysis including Rh incompatibility.  The aim of this study was to assess the rate of hyperbilirubinemia due to hemolysis in Rh positive neonates  born to Rh negative mothers. Methods In this retrospective study from March 1998 to February 2003, 60 icteric Rh positive newborns that were born to Rh negative m...

The influence of temperature and relative air humidity on the stability of ceftriaxone disodium (CTN) in Biotrakson and Tartriakson was investigated. At RH = 0%, the degradation of ceftriaxone was the first-order reaction, while at RH from 50.9% to 76.4% it was an autocatalytic first-order reaction relative to the substrate concentration. The influence of temperature on the stability of CTN was...

Several researchers have shown that Pt-Rh bimetallic catalysts can perform synergistically for the NO/H2, NO/CO, CO/O2, and NO/CO/HC reactions [1-5]. Lakis and coworkers clarified some of the mystery surrounding the synergistic performance of Pt-Rh catalysts for the NO + H2 reaction by using analytical electron microscopy (AEM) to show that the synergistic performance of a Pt(95%)Rh(5%)/γ-Al2O3...