OBJECTIVE(S) Staphylococcus aureus is both a successful human commensal and a major pathogen. In this study we investigated the genetic diversity of 26 S. aureus isolates recovered from human skin and urinary tract infections. MATERIALS AND METHODS Typing procedure for the studied S. aureus isolates was performed based on PCR amplification of the aroA gene, which encodes the enzyme 5-enolpyru...

A polyphasic approach was applied to characterize 35 G. diazotrophicus isolates obtained from sugarcane varieties cultivated in Brazil. The isolates were analyzed by phenotypic (use of different carbon sources) and genotypic tests (ARDRA and RISA-RFLP techniques). Variability among the isolates was observed in relation to the carbon source use preference. Glucose and sucrose were used by all is...

To study genetic diversity of Cryptococcus neoformans species complex in Spain, 97 isolates of the yeast recovered from human, animal and environmental samples have been analysed using three molecular epidemiological techniques. One of these, URA5 gene fragment length polymorphism (RFLP) analysis, has been previously described as a molecular epidemiology tool. Thus, standard profiles and refere...

The most common form of the disease is cutaneous leishmaniasis (CL) which is a public health and social problem in many countries especially Iran. In endemic areas where other diseases with similar clinical symptoms occur, definitive diagnosis of CL is very important. The detection and identification of Leishmania in infected patients is crucial for achieving a correct treatment and prognosis. ...

introduction: candida dubliniensis and c. albicans are very similar in morphology and phenotypic characteristics. approximation of this yeast species has caused major problems in identifying these two correctly. materials and methods: to distinguish among sixty yeast clinical isolates from patients with cancer, polymerase chain reaction - restriction fragment length polymorphism (pcr-rflp) was ...

Various chromosomal banding techniques were utilized on the catfish, Iheringichthys labrosus, taken from the Capivara Reservoir. C-banding regions were evidenced in telomeric regions of most of the chromosomes. The B microchromosome appeared totally heterochromatic. The restriction endonuclease AluI produced a banding pattern similar to C-banding in some chromosomes; the B microchromosome, when...

introduction : streptococcus pneumoniae cause morbidity and mortality in infants and younger children. because of high prevalence of penicillin resistance, rapid and reliable diagnostic techniques for penicillin non-susceptible s. pneumoniae (pnssp) are important for prevention and treatment. we investigated the association of the restriction length polymorphism (rflp) patterns for pbp2b to dis...

The aim of the present study was to detect polymorphism in Insulin like growth factor-I (IGF-I) gene of beluga (Huso huso) fish using PCR-SSCP technique and also investigation of its association with growth traits (condition factor, body length and weight). A total of 150 specimens of beluga were randomly selected and DNA was isolated from caudal fin using modified salting out method. Then two ...