نتایج جستجو برای: reverse transcriptase loop mediated isothermal amplification assay

تعداد نتایج: 876614  

Journal: :International Journal of Current Microbiology and Applied Sciences 2020

Journal: :Estudos 2022

A técnica de Loop Mediated Isothermal Amplification Assay (LAMP) amplamente difundida a partir dos anos 2000, baseia-se na utilização primers sensíveis e específicos da BST DNA polimerase junto à outros reagentes para amplificação uma sequência alvo sob temperatura constante. Durante LAMP ocorrem sucessivos ciclos etapa não cíclica produção exponencial amplicons comprimentos variáveis. Esses pr...

2017
Amanda E Calvert Brad J Biggerstaff Nathan A Tanner Molly Lauterbach Robert S Lanciotti

Zika virus (ZIKV) has emerged as a major global public health concern in the last two years due to its link as a causative agent of human birth defects. Its rapid expansion into the Western Hemisphere as well as the ability to be transmitted from mother to fetus, through sexual transmission and possibly through blood transfusions has increased the need for a rapid and expansive public health re...

2012
Kelly A. Curtis Donna L. Rudolph Irene Nejad Jered Singleton Andy Beddoe Bernhard Weigl Paul LaBarre S. Michele Owen

BACKGROUND To date, the use of traditional nucleic acid amplification tests (NAAT) for detection of HIV-1 DNA or RNA has been restricted to laboratory settings due to time, equipment, and technical expertise requirements. The availability of a rapid NAAT with applicability for resource-limited or point-of-care (POC) settings would fill a great need in HIV diagnostics, allowing for timely diagno...

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