A novel technique to estimate the distribution state of carboxyl groups within submicron-sized, carboxylated polymer particles was proposed. For the purpose, carboxyl groups in two kinds of butyl methacrylate-methacrylic acid copolymer emulsion, which were prepared by emulsion copolymerizations with different monomer addition methods, were titrated by isothermal titration calorimetry with an ex...

Droplet microfluidics, in which micro-droplets serve as individual reactors, has enabled a range of high-throughput biochemical processes.[1] The talk will start with our recent application on using droplets to identify bacteria, specifically methane-metabolizing bacteria, for increasing the efficiency of generation of bioplastics.[2] This work, along with emerging applications to screen very l...

In situ gelatin zymography is a simple technique providing valuable information about the cellular and tissue localization of gelatinases. Until recently, the use of this technique has been confined to soft, relatively homogeneous tissue. In this report in situ zymography has been utilized to assess the sub-lamellar location of gelatinases in the hard, semi-keratinized epidermal layer and the a...

Reassociating double-stranded DNA from single-stranded components is necessary for many molecular genetics experiments. The choice of a DNA reassociation method is dictated by the complexity of the starting material. Reassociation of simple oligomers needs only slow cooling in an aqueous environment, whereas reannealing the many single-stranded DNAs of complex genomic mixtures requires both a p...

Autoradiographic techniques for use with the electron microscope have been developed and considerably improved in recent years. A review of this historical development is given by Meek and Moses (14), and a theoretical analysis of the problems involved by Pelc (17) and Caro (4). In this paper, we describe a method for preparing autoradiographic specimens, using a new, fine-grained emulsion and ...

Autoradiographic techniques for use with the electron microscope have been developed and considerably improved in recent years. A review of this historical development is given by Meek and Moses (14), and a theoretical analysis of the problems involved by Pelc (17) and Caro (4). In this paper, we describe a method for preparing autoradiographic specimens, using a new, fine-grained emulsion and ...