More than 80% of the total milk protein contains caseins that are to forms α-s1, α-s2, β-casein and kappa casein. Kappa casein is smaller than other milk proteins but due to have a role in size and stability of micelles. The present study describes polymorphism of kappa casein gene. This is the first study of kappa casein gene polymorphism in Najdi cattle of Iran. We used the polymerase chain r...

Terminal restriction fragment length polymorphism (T-RFLP) analysis has the potential to be useful for comparisons of complex bacterial communities, especially to detect changes in community structure in response to different variables. To do this successfully, systematic variations have to be detected above method-associated noise, by standardizing data sets and assigning confidence estimates ...

thirtyfrieswal lactating cows were screened for clinical and subclinical mastitis and subsequently classified into healthy, subclinically affected and clinically affected groups each group comprising of 10 cows. polymorphism of cow lactoferrin (ltf) gene promoter was determined by polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp). the results showed that lft gene pro...

We describe a modified single nucleotide polymorphism (SNP) typing method based on the restriction fragment length polymorphism polymerase chain reaction (RFLP-PCR). This is a simple, economical method without the need for special equipment. For most SNP loci, a common restriction endonuclease (Hind III, EcoR I or BamH I) recognizing site (RER) can be introduced into one allelic form, but not t...

Thesis Abstract Several cytokines are known to be involved in the pathogenesis of non-Hodgkin Lymphomas (NHL). Given importance understanding genetic predisposition NHL, this work was designed study impact IL-10 (-1082 G/A; rs1800896 and -819 C/T; rs1800871), IL-6 (-174 G/C; rs1800795) CD38 (184C/G; rs6449182) gene polymorphisms on susceptibility Egyptians NHL. Both loci IL10 were genotyped usi...

A highly efficient, rapid, and reliable PCR-based method for distinguishing Lactococcus lactis subspecies (L. lactis subsp. lactis and L. lactis subsp. cremoris) is described. Primers complementary to positions in the glutamate decarboxylase gene have been constructed. PCR analysis with extracted DNA or with cells of different L. lactis strains resulted in specific fragments. The length polymor...