Background: Male infertility is responsible for approximately 50% of infertility in the world. Reactive oxygen species (ROS) is one of the causative agents of infertility in males which effects on sperm quality and function. In this study, the effects of oxidative stress induced by tertiary-butyl hydroperoxide (TBHP) were investigated on sperm quality, testis tissue and miRNAs expression. Mater...

Background: Prolonged alcohol abuse in men can cause testosterone deficiency and shrinkage of the testicles (testicular atrophy). This can lead to impotence, sterility and ‘feminisation’. When alcohol is broken down in the body, it may also interfere with normal sperm structure and movement. This study was designed to determine the effects of administration of alcohol on sperm quality, sperm ma...

Background: Cholesterol loaded cyclodextrin (CLC) efficiently protected sperm from some species. On the other hand vitrification of sperm especially in human opened a new horizon in the field of sperm freezing. The aim of the present study was to evaluate CLC on vitrification of cat sperm. Materials and Methods: Tom epididymal sperm (n=9) was extracted from epididymides and subjected to vitrifi...

Background: Formaldehyde is widely used in industry and in medicine as tissue fixative and disinfectant. It contains reactive molecules which have been known for its cytotoxic effects. In the present study, the probable protective properties of Rosa damacena aquatic extract, against the harmful effects of formaldehyde was evaluated by consideration of sperm count, motility, viability, morpholog...

s of papers 107 was added to PL at 10 and 20 mol% (PL10, PL20). PL10 enhanced fusion (82.4 ± 1.4 vs. 58.4 ± 3.0% sperm fused, PL10 vs. PL20, n = 7; P < 0.0001) and sperm viability (27 ± 2, 46 ± 3, and 39 ± 4%, respectively, for PL, PL10, and PL20, P < 0.05). Because viability was still unacceptably low, we then added poly-L-lysine to the PL10 recipe at 1 (PL10−1), 5 (PL10−5), and 10 mol% (PL10−...

Internal fertilization requires live sperm to be transferred from male to female before egg fertilization. Both males and females assist the insemination process by providing sperm with glandular secretions, which have been inferred to contain subsets of proteins that maintain sperm viability. Here we show that in the honeybee (Apis mellifera) secretions of the male accessory glands, the major ...

Background: Formaldehyde (FA, H2CO), a member of the aldehyde family and one of the simplest organic molecules, is a flammable, pungent, irritating and colorless gas. This study aimed to investigate the effect of formaldehyde (FA) vapor on sperm parameters and testicular tissue concern about the duration of analysis and the amount of the formaldehyde vapor concentrations. Materials and Methods:...

In this study, we conducted a simultaneous analysis of sperm count and viability in rats by flow cytometry (FCM). Epididymal fluids were taken from the caudal epididymis of 12 to 13 week-old Sprague-Dawley rats. The fluids were weighed and mixed with Dulbecco's phosphate buffered saline (D-PBS). Propidium iodide, which can stain only dead sperm, was used to distinguish viable and dead sperm. Th...