AIMS In order to improve the diagnosis of Bacillus anthracis in environmental samples, we established a DNA microarray based on the ArrayTube technology of Clondiag. METHODS AND RESULTS Total DNA of a bacterial colony is randomly biotinylated and hybridized to the array. The probes on the array target the virulence genes, the genomic marker gene rpoB, as well as the selective 16S rDNA sequenc...

INTRODUCTION Bacillus anthracis is the causative agent of anthrax, a disease endemic in regions of Northern Cape Province and Kruger National Park of South Africa. Accurate identification of virulent B. anthracis is essential but challenging due to its close relationship with other members of B. cereus group. This study characterized B. anthracis and Bacillus species that were recovered from an...

This study investigates the potential of epiphytic bacteria isolated from citrus fruits to control green mold post-harvest disease caused by Penicillium digitatum in Thompson Navel sweet oranges north Iran. Twenty-three were and screened vitro against P. digitatum, 13 isolates with antagonistic activity selected for further studies. Isolates B15 (Bacillus sp.), P17 (Pseudomonas S13 (Streptomyce...

OBJECTIVE(S) We aimed to characterize the phenotype and genotype of Bacillus spp isolated from diabetic patients' eyes, by studying the drug sensitivity patterns with a disc-diffusion method. MATERIALS AND METHODS Fifty eyes of 25 patients with type II diabetes mellitus, with at least 10 years of diabetes history, were included in the study. We analyzed the eyes for the presence of Bacillus s...

A broth dilution assay was used to determine the in vitro susceptibilities of 10 ocular isolates of Bacillus cereus to clindamycin, gentamicin, and vancomycin both alone and in combination. The checkerboard technique was used to determine fractional inhibitory and bactericidal concentration indices for combinations of clindamycin-gentamicin and vancomycin-gentamicin.

Andaman and Nicobar Islands, considered as one of the important marine ecosystem in India and being considered as the richest source for microbial community. The marine microbial taxa and actinomycetes diversity are not been fully explored. Actinomycetes were isolated from marine soil samples and screened for antibacterial activity. Out of eight isolates, the isolate AN1 isolated from soil samp...

Bacterial species present in the Berg and Plankenburg Rivers (Western Cape, South Africa) were isolated from water and biofilm samples and population shifts between sampling sites were phylogenetically identified. Deoxyribonucleic acid (DNA) extraction of representative isolates was performed and amplified using 2 different primer sets. Various Enterobacteriaceae species were present at all of ...

background and objectives: biosurfactants are surface active agents with broad range commercial applications in various industries and have considerable advantages over their chemical counterparts. materials and methods: in this study, bacteria were isolated from contaminated and uncontaminated soil and selected during preliminary screening using hemolytic activity, oil spreading and oil collap...