نتایج جستجو برای: reporter gene assay
تعداد نتایج: 1313861 فیلتر نتایج به سال:
Abstract Background and Aims Peritoneal dialysis (PD) as a long-term renal replacement therapy is limited by peritoneal fibrosis. The purpose of our study was to identify the mechanism mesenchymal stem cell-derived exosomes (MSC-Exos) in regulating transdifferentiation fibroblasts (FBs) myofibroblasts (MFBs) dialysis-associated Method experimental mice were divided into 3 groups according treat...
Intracellular calcium response and resulting calcium signaling to an agonist-GPCR interaction are important for the measurement of compound activity in the GPCR drug development. The increase in cytosol calcium concentration can be measured by the fluorescent calcium indicator dye such as Fluo-4 in a quick assay (in 3-5 minutes) using the fluorescence imaging plate reader. The calcium signaling...
Combinatorial gene inactivation using an RNAi library is a powerful approach to discovering novel functional genes. However, generation of a comprehensive RNAi library remains technically challenging. In this report, we describe a simple and novel approach to designing gene-family-specific RNAi libraries by targeting conserved motifs using degenerate oligonucleotides. We created an siRNA librar...
Induction of cytochrome P450 3A4 (CYP3A4) is determined typically by employing primary culture of human hepatocytes and measuring CYP3A4 mRNA, protein and microsomal activity. Recently a pregnane X receptor (PXR) reporter gene assay was established to screen CYP3A4 inducers. To evaluate results from the PXR reporter gene assay with those from the aforementioned conventional assays, 14 drugs wer...
Abstract The multifunctionality of genome is suggested at some loci in different species but not well understood. Here we identified a DES-K16 region an intron the Kctd16 gene as chromatin highly marked with epigenetic modifications both enhancers (H3K4me1 and H3K27ac) silencers (H3K27me3) mouse spermatocytes. In vitro reporter assay demonstrated that exhibited significant enhancer activity spe...
In the Gal4 adaptor assay, leucine zipper-containing proteins are tethered indirectly to the promoters of Gal4-responsive reporter genes via synthetic protein chimeras consisting of a Gal4 DNA-binding domain with an attached leucine zipper. Ternary complexes composed of the DNA binding site, the adaptor protein, and a leucine zipper factor can stimulate reporter gene activity, provided that the...
To study the biology of regulators of G-protein signaling (RGS) proteins and to facilitate the identification of small molecule modulators of RGS proteins, the authors recently developed an advanced yeast 2-hybrid (YTH) assay format for GalphaZ and RGS-Z1. Moreover, they describe the development of a multiplexed luciferase-based assay that has been successfully adapted to screen large numbers o...
The fat content of milk determines the quality milk, and triglycerides are major components fat. Milk synthesis is regulated by many factors. Lipopolysaccharide (LPS) has been shown to inhibit in bovine mammary epithelial cells, but research on underlying mechanisms limited. MicroRNA (miRNA) involved physiological processes, there have few studies their regulation synthesis. In this study, we a...
The indolizidine alkaloid-swainsonine (SW) is the main toxic component of locoweeds and cause locoweed poisoning in grazing animals. endophytic fungi, Alternaria Section Undifilum spp., are responsible for biosynthesis SW locoweeds. swnK gene a multifunctional complex enzyme encoding fungal biosynthesis, its product plays key role multistep catalytic synthesis by fungi using pipecolic acid as p...
Reduced host cell reactivation (HCR) of a reporter gene containing 8-oxoguanine (8-oxoG) lesions in Cockayne syndrome (CS) fibroblasts has previously been attributed to increased 8-oxoG-mediated inhibition of transcription resulting from a deficiency in repair. This interpretation has been challenged by a report suggesting reduced expression from an 8-oxoG containing reporter gene occurs in all...
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