We have constructed a RP4 KorB overproducing strain and purified the protein to near homogeneity. KorB is a DNA binding protein recognizing defined palindromic 13-bp sequences (TTTAGCSGCTAAA). Inverted sequence repetitions of this type, designated OB, are present on RP4 12 times. OB-sequences are localized in replication and maintenance regions as well as in the regions Tra1 and Tra2 essential ...

Since the introduction of nominative signature in 1996, there have been only a few schemes proposed and all of them have already been found flawed. In addition, there is no formal security model defined. Even more problematic, there is no convincing application proposed. Due to these problems, the research of nominative signature has almost stalled and it is unknown if a secure nominative signa...

The structural domains of human apolipoprotein B-100 in low density lipoproteins (LDL) and the conformational changes of B-100 that accompany the conversion of very low density lipoproteins (VLDL) to LDL were investigated by limited proteolysis with 12 endoproteases of various specificities, and their cleavage sites were determined. In B-100 of LDL, we identified two peptide regions that are hi...

This article is devoted to the consideration of methods for assessing tax potential regions, as well factors impacting potential. The aim research review approaches designated determining essence concept, study influencing thereupon. In process regions in analysis, we use data on budget revenues by region 2008-2020, regional GDP, investments fixed assets, arrears and number operating taxpayers....

Objective: A study of primary cancer morbidity among children and subsequent calculation of average annual incidence were carried out for boys and girls, and young men and women in Kazakhstan. Methods: The investigated population lived in three areas of the Aral Sea region: designated catastrophe (Aral, Kazalt, Shalkar regions), crisis (Zhalagash, Karmakshy, Shiely regions), pre-crisis (Irgiz, ...

From 1 to 28 days after frog sciatic nerve damage, dorsal root ganglia were incubated with [35S] methionine, and the labeled, rapidly transported proteins at various points along the nerve were analyzed on two-dimensional gels. The results show a dramatic increase in the labeling of a protein, which we have designated as A25, only after the arrival of the rapidly transported proteins at regener...

The last large-scale ecotoxicological study of ospreys (Pandion haliaetus) in Chesapeake Bay was conducted in 2000-2001 and focused on U.S. EPA-designated Regions of Concern (ROCs; Baltimore Harbor/Patapsco, Anacostia/middle Potomac, and Elizabeth Rivers). In 2011-2012, ROCs were re-evaluated to determine spatial and temporal trends in productivity and contaminants. Concentrations of p,p'-DDE w...

Glocalization as a concept; is to adapt global products or values local cultures. For example, while using generally accepted design elements in the interior designs of chain hotels, which are businesses, glocalization effects also observed. This study aims determine made context lobbies where hotels create first impressions customers. this purpose, literature review was conducted. The Marriott...