An efficient plant regeneration protocol was developed from leaf and internode (stem) explants of Bacopa monniera L. on MS basal medium, supplemented with Cytokinins like BAP, KN, and TDZ used singly. The leaf cultures expressed maximum plant regeneration potential with 138.9 ± 0.50 shoots per explants on MS + 2.0 mg/ l BAP. The in vitro regenerated dwarf shoots were further elongated on MS med...

An in vitro regeneration protocol through stem callus culture has been standardized for the medicinal climber Clematis gouriana. The explant induced callus on MS-medium supplemented with 0.5 to 1.5 mg l BAP and 0.1 to 0.5 mg l NAA. The optimized callus induction occurred at the concentration of 1.0 mg l BAP and 0.3 mg l NAA. After initiation of callus, it was immediately transferred to MS mediu...

Callus induction and regeneration in vitro of three rice varieties (Oryza sativa L. ssp indica), Bg 94-1, Moraberekon and Bg 300 were examined. Phenotypic variations of regenerated and parent plants were compared and the method of plant regeneration was confirmed by histology. Callusing was induced by culturing the mature embryos of the three rice varieties in a liquid medium of distilled water...

of embryogenic frequency 88.9 and 72.23 were recorded for stem explants. However, the greatest embryogenic calli fresh weights 14.68 and 9.65 (mg/jar) were derived from leaf explants. Moreover, growth of somatic embryos was investigated during 7weeks. The highest regeneration percentage was achieved for stem explants compared other explants and recorded 88.88 and 66.66 for directly or indirectl...

In this report we describe a methodology for rapid sugarcane shoot regeneration using meristematic tissue from in vitro-grown plantlets. Shoot regeneration from previously induced meristematic tissue, in the presence of up to 22.62 μM of 2,4-D was evaluated. The explants were transferred, after a week induction, to a propagation medium supplemented with activated charcoal and then transferred t...

An efficient method for in vitro micro propagation and genetic transformation of plants are crucial for both basic and applied research. Maize is one of the most important cereal crops around the world. Regeneration from immature embryo is hampered due to its unavailability round the year. On the contrary mature embryo especially tropical maize is recalcitrant toward tissue culture. Here we rep...

Background & Aim: Dracocephalum genus belongs to the Lamiaceae family which has eight species in Iran. Dracocephalum kotschyi Boiss is an important medicinal and aromatic plant that use in pharmaceutical industry. In addition, this species is used in Iranian traditional medicine. The aim of current study was to examine the various growth regulations and explants on direct orga...

Background & Aim: Dracocephalum genus belongs to the Lamiaceae family which has eight species in Iran. Dracocephalum kotschyi Boiss is an important medicinal and aromatic plant that use in pharmaceutical industry. In addition, this species is used in Iranian traditional medicine. The aim of current study was to examine the various growth regulations and explants on direct orga...