long-term protection against hepatitis b virus (hbv) is dependent on persistence of anti-hbs antibodies and/or strong immunological memory. in this study we evaluated the persistence of anti-hbs antibodies in healthy iranian children 5 years after primary vaccination and the response to a booster dose using recombinant hepatitis b vaccine. totally, 81 children who had received primary course of...

introduction: the development of an effective vaccine against hepatitis c virus (hcv) is still a target of intensive vaccine research. the hcv envelope proteins e1 and e2 which can induce broadly neutralizing antibodies are the major candidate for this purpose. different types of expression systems have been used to express these glycoproteins. in this study, an expression system using pichia p...

background: 1.3% of people in iran are positive for anti-hbc antibody.the objective of this study is to determine the rate of seroconversion in those with persistent isolated anti-hbc after vaccination. materials and methods: in a quasi-experimental study, 94 persons with isolated anti-hbc antibody who were referred to tehran blood transfusion organization between 2002 and 2004 were selected th...

objectives: enterotoxigenic escherichia coli (etec) is one of the most common agents of diarrhea among other bacterial agents. designing and producing vaccine against these bacteria is one of the major purposes of world health organization (who). due to presence of diverse clones of etec strains in the world, the use of global vaccines for etec infection is controversial. b subunit of heat labi...

Objective(s):Multi-epitopic protein vaccines and direction of vaccine delivery to dendritic cells (DCs) are promising approaches for enhancing immune responses against mutable pathogens. Escherichia coli is current host for expression of recombinant proteins, and it is important to optimize expression condition. The aim of this study was the optimization of multi-epitopic HIV-1 tat/pol/gag/env ...

Various vaccine platforms are geared against COVID-19 development to produce immunogens in cells. To design a recombinant protein-based vaccine, Vaxine pty Ltd used computer models of the spike protein and its human receptor, ACE2, identify how virus infects Based on this, COVAX-19? is synthesized. It does reduce not only disease but also blocks shedding transmission. Researchers optimistic tha...

Avian Pasteurella multocida is the causative agent of fowl cholera, a disease much affecting the poultry industry. In order to study the efficacy of the recombinant subunit vaccine constructed with ptfA gene of avian P. multocida, the ptfA gene fragment amplified by PCR from avian P. multocida was cloned into the prokaryotic expression vector pET32a and the recombinant plasmid pET32a-ptfA was o...

Mycobacterium bovis BCG is the only available vaccine for protection against tuberculosis (TB). While BCG protects children from severe disease, it has little impact on pulmonary disease in adults. A recombinant BCG vaccine BCG ΔureC::hly (strain VPM1002) is in advanced clinical trials and shows promise for improved vaccine safety but little change in efficacy in animal models. A second-generat...

Background: Tuberculosis as a global health problem requires special attention in the contexts of prevention and control. Subunit vaccines are promising strategies to protect burdens of tuberculosis via increasing the BCG protection. The present study aimed to design a vaccine study by means of high-throughput expression and correct refolding of recombinant protein PPE17. Methods: We aimed to c...

We have recently developed a transmissible vaccine to immunize rabbits against myxomatosis and rabbit haemorrhagic disease based on a recombinant myxoma virus (MV) expressing the rabbit haemorrhagic disease virus (RHDV) capsid protein [Bárcena et al. Horizontal transmissible protection against myxomatosis and rabbit haemorragic disease using a recombinant myxoma virus. J. Virol. 2000;74:1114-23...