l-DOPA is a major drug for the treatment of Parkinson’s disease, and microbial enzymatic synthesis an essential way its industrial production. The expression tyrosine phenol-lyase (TPL) derived from Erwinia herbicola under six different constitutive promoters in Escherichia coli was studied. recombinant strain 5 can express TPL well with growth. optimal medium composition this as follows: trypt...

conclusions etec and eaggec were the most detected e. coli among stool and uti samples, emphasizing the need to dedicate more health care attention to this group. in addition, our phylogenetic study may be helpful in figuring out the infection origin and for epidemiological studies. nonetheless, more research studies with larger sample sizes are suggested for confirming our results. results ove...

Lactoferrin is a non-hematic iron-binding 80-kDa protein that exhibits antimicrobial activity. Higher plants function as “green bioreactors” for large-scale recombinant production. In this study, we transiently expressed human lactoferrin (rhLF) in Nicotiana benthamiana at yield of approximately 40 µg g−1 fresh mass (gFM) using the Tsukuba system. Additionally, expression level rhLF increased w...

The production of hydrogen via microbial biotechnology is an active field of research. Given its ease of manipulation, the best-studied bacterium Escherichia coli has become a workhorse for enhanced hydrogen production through metabolic engineering, heterologous gene expression, adaptive evolution, and protein engineering. Herein, the utility of E. coli strains to produce hydrogen, via native h...

enterohemorrhagic escherichia coli (ehec) of the o157:h7 serotype is a worldwide zoonotic pathogen responsible for the majority of severe cases of human ehec disease. the aim of the present study was to investigate the prevalence of e. coli o157: h7/nm in raw meat samples from two provinces of iran. during a period from march 2010 to march 2011. two hundred and ninety five raw meat samples were...

Escherichia coli is one of the most widely used hosts for the production of heterologous proteins and its genetics are far better characterized than those of any other microorganism. Recent progress in the fundamental understanding of transcription, translation, and protein folding in E. coli, together with serendipitous discoveries and the availability of improved genetic tools are making this...

The secretory production of recombinant proteins by the Gram-negative bacterium Escherichia coli has several advantages over intracellular production as inclusion bodies. In most cases, targeting protein to the periplasmic space or to the culture medium facilitates downstream processing, folding, and in vivo stability, enabling the production of soluble and biologically active proteins at a red...

The bacterial species Escherichia coli has proven to be a powerful tool in the molecular analysis of polyhydroxyalkanoate (PHA) biosynthesis. In addition, E. coli holds promise as a source for economical PHA production. Using this microorganism, clones have been developed in our laboratory which direct the synthesis of poly-beta-hydroxybutyrate (PHB) to levels as high as 95% of the cell dry wei...