background helicobacter pylori multiplies and causes infection in human gastric mucosal layer. new approaches have focused on using specific treatments, such as immunotherapy, to limit this infection. urease, as one of the most important virulent and antigenic factors of the bacterium, is a suitable target for this purpose. patients and methods in order to prepare recombinant proteins, the synt...

taq dna polymerase is widely used in laboratories and for this reason many investigators have focused their attention on understanding the role of various regions and amino acids in this enzyme. o-helix is a part of taq polymerase suggested to play an important role in the enzyme fidelity. the influence of asn666 in this helix on the enzyme function has never been investigated, and therefore by...

helicobacter pylori infection is among the most prevalent infections in the world involving more than half the adult population. h. pylori infection results in active chronic gastritis, peptic ulcers and enhances the risk of gastric malignancies. it is of utmost importance to prevent h. pylori infection particularly in highly prevalent countries including iran. the urease holoenzyme produced by...

background: staphylococcus aureus (s. aureus) is a major nosocomial pathogen and the infection with this organism in human is increasing due to the spread of antibiotic resistant strains. one of the resistance mechanisms of s. aureus comprises modification in binding proteins to penicillin. vaccine strategy may be useful in controlling the infections induced by this organism. this study aimed a...

background: recombinant vaccine technology is one of the most developed means in controlling infectious diseases. however, an effective vaccine against shigella is still missing. objective: to evaluate recombinant ipac protein of shigella as a vaccine candidate. methods: in this study we cloned ipac gene into an expression vector in prokaryotic system. the protein expression was evaluated by sd...

Objective(s): Vascular endothelial growth factor (VEGF) is one of the most effective proteins in angiogenesis, mesenchymal stem cells (MSCs) differentiation and wound healing. These abilities are therapeutic potential of VEGF in diabetic retinopathy, nephropathy and other tissue damage circumstances. In this study, recombinant VEGF was produced in Escherichia coli (E. coli) system and then biol...

  Objective(s): Streptokinase (SKa) is an antigenic protein which is secreted by Streptococcus pyogenes. Streptokinase induces inflammation by complement activation, which may play a role in post infectious diseases. In the present study, recombinant streptokinase from S. pyogenes was produced and showed that recombinant SKa protein was recognized by infected human sera using Western blot analy...