Mucopolysaccharidosis (MPS) I is a lysosomal storage disease caused by a deficiency of α-L-iduronidase (IDUA) (EC 3.2.1.76); enzyme replacement therapy is the conventional treatment for this genetic disease. Arabidopsis cgl mutants are characterized by a deficiency of the activity of N-acetylglucosaminyl transferase I (EC 2.4.1.101), the first enzyme in the pathway of hybrid and complex N-glyca...

Objective(s): Helicobacter pylori, a human specific gastric pathogen is a causative agent of chronic active gastritis. The vacuolating cytotoxin (VacA) is an effective virulence factor involved in gastric injury. The aim of this study was to construct a recombinant protein containing antigenic region of VacA gene and determine its antigenicity.   Materials and Methods: The antigenic region of V...

BACKGROUND We have previously reported on purification and characterization of beta-fructofuranosidase (beta-FFase) from Bifidobacterium adolescentis G1. This enzyme showed high activity of hydrolysis on fructo-oligosaccharides with a low degree of polymerization. Recently, genome sequences of B. longum NCC2705 and B. adolescentis ATCC 15703 were determined, and cscA gene in the both genome seq...

Background: The critical role of interleukin-7 (IL-7) in homeostatic proliferation and T cell survival has made it a promising cytokine for the treatment of various clinical conditions, especially those associated with lymphopenia. Methods: In the present study we expressed recombinant human interleukin-7 (rhIL-7) in Chinese hamster ovary (CHO)-K1 cells. CHO-K1 cells were stably transfected ...

Introduction: Multiple sclerosis (MS) is a disease of the immune system: it attacks the myelin around the axons and leaves them exposed. Destruction of myelin weakens the electrical conduction of ions and thus leads to a lack of communication in the nervous system.  Methods: In the present study, we constructed recombinant plasmid and then transformed to E. coli cell. The colonies containing...

Recently, we described the preparation of recombinant oleate hydratase from Lactobacillus rhamnosus ATCC 53103. We observed that purified C-terminal His-tagged enzyme was completely inactive and catalytic activity partially restored only in presence a large amount flavin adenine dinucleotide (FAD). In present work, assess this reduced form (FADH2) is at least one hundred times as active same co...

The hippurate hydrolase enzyme of Campylobacter jejuni was expressed in Escherichia coli as a six-histidine-tagged fusion protein. The purified recombinant enzyme was characterized to gain an understanding of the structure and activity of the hippurate hydrolase. The recombinant enzyme had a native molecular mass of 193+/- 11 kDa a reduced molecular mass of 42.4+/- 0.8 kDa, and possessed 1.98+/...

Growing applications of phenylalanine dehydrogenase (PheDH) enzyme in the medical and pharmaceutical industries encourages researchers to seek simple, fast and economical alternative purification methods. With goal of finding a new technique, the extraction and purification of recombinant Bacillus badius PheDH in polyethylene glycol 6000 (PEG-6000) and ammonium sulfate aqueous two-phase systems...

Mucopolysaccharidosis IV A (MPS IV A, Morquio A disease) is a lysosomal storage disease (LSD) produced by mutations on N-acetylgalactosamine-6-sulfate sulfatase (GALNS). Recently an enzyme replacement therapy (ERT) for this disease was approved using a recombinant enzyme produced in CHO cells. Previously, we reported the production of an active GALNS enzyme in Escherichia coli that showed simil...

Alcanivorax borkumensis is a marine bacterium that has ability to grow on limited substrates that mainly is alkanes. The ability to use wide range of hydrocarbons is advantage of this bacterium to other marine community bacteria. A. borkumensis have two genetic systems for alkane biodegradation. The First system is alkane hydroxylase (alk-B1and alk-B2) and the second system is...