Abstract Background and Aims Poly(ADP-ribosy)lation (PARylation), which is mediated by poly(ADP-ribose) polymerases (PARPs), catalyzes the transfer of ADP-ribose from NAD+ molecules to acceptor proteins, regulates diverse cellular processes. Since PARP1 gene-deficient mice revealed an increase in urine volume, we aimed examine role PARP1, most abundant type protein PARPs family, vasopressin-med...

Adenoviral gene transfer is a valuable tool in molecular biology research. In order to be an efficient and safe vector, adenovirus structure and infection mechanism as well as molecular biology of the used transgene need to be well studied. The aim of this study was to evaluate the role of adenovirus as a gene transfer vector from several perspectives. Adenovirus uses receptor-mediated endocyto...

background: nephrotic syndrome is a disorder that leads to hyperlipidemia. l-carnitine and genistein can effect on lipid metabolism and the syndrome. in the present study, we have delved into the separate and the twin-effects of l-carnitine and genistein on the gene expressions of hmg-coa reductase and ldl receptor in experimental nephrotic syndrome. methods: in this controlled experimental stu...

melatonin regulates some major physiological processes such as maturation and function of reproductive system, pubertal development, seasonal reproduction and adaptation. the activation of melatonin hormone is mediated by melatonin receptor. previous studies showed melatonin receptor 1a gene (mtnr1a) is highly polymorphic in seasonally breeding species. the aim of the present study was detectio...

DNA-mediated gene transfer is a procedure which uses purified DNA to introduce new genetic elements into cells in culture. The standard DNA-mediated gene transfer procedure involves the use of whole cell DNA as carrier DNA for the transfer. We have modified the standard DNA-mediated gene transfer procedure to transfer the Herpes simplex virus type 1 thymidine kinase gene (TK) into TK- murine re...

Gene therapy in the liver requires powerful vectors capable of mediating sufficient gene delivery and expression in affected hepatocytes. Viral vectors are amongst the most efficient tools for gene delivery, and the search for tissue-specific infecting viruses is important for the development of in v i v o gene therapy strategies. We have recently shown for the first time that a genetically mod...