Here we describe lentivirus-infected cell microarrays for the high-throughput screening of gene function in mammalian cells. To create these arrays, we cultured mammalian cells on glass slides ‘printed’ with lentiviruses pseudotyped as vesicular stomatitis virus glycoprotein, which encode short hairpin RNA or cDNA. Cells that land on the printed ‘features’ become infected with lentivirus, creat...

Genetically encoded photosensitizers, proteins that produce reactive oxygen species when illuminated with visible light, are increasingly used as optogenetic tools. Their applications range from ablation of specific cell populations to precise optical inactivation of cellular proteins. Here, we report an orange mutant of red fluorescent protein KillerRed that becomes toxic when illuminated with...

The synthesis of two new azobenzene dyes, namely CR-528 and CR-555, and their spectral properties in ethanol solution are described. The recognition of sulfur-containing analytes (2-mercaptoethanol (2-ME), sodium hydrosulfide (NaHS)), and biogenic amines (spermine, spermidine, ethanolamine) bestowed significant spectral changes with color changes from pink/purple to pale yellow/orange-yellow. T...

The central nervous system (CNS) has very restricted intrinsic regeneration ability under the injury or disease condition. Innovative repair strategies, therefore, are urgently needed to facilitate tissue regeneration and functional recovery. The published tissue repair/regeneration strategies, such as cell and/or drug delivery, has been demonstrated to have some therapeutic effects on experime...

[F]DPA-714 [N,N-diethyl-2-(2-(4-(2[F]-fluoroethoxy)phenyl) 5,7dimethylpyrazolo[1,5a]pyrimidin-3-yl)acetamide] is a new radioligand currently used for imaging the 18-kDa translocator protein in animal models of neuroinflammation and recently in humans. The biodistribution by positron emission tomography (PET) in baboons and the in vitro and in vivo metabolism of [F]DPA-714 were investigated in r...

Aim. A new manufacturing method aiming at to producing more flexible and resistant NiTi endodontic instruments has been recently developed (Hyflex, produced with CM wire). The purpose of the study was to determine whether this new manufacturing method produces NiTi instruments (Hyflex) of superior flexibility and/or superior resistance to cyclic fatigue, when compared with instruments produced ...

the aim of this study was to determine the inhibitory effects of feed ingredients on protease activities of argyrosomus regius larvae using in vitro techniques. a. regius larvae fed on a commercial feeding procedure were sampled thirteen times, during the sampling period (from 3 to 32 days after hatching (dah)). the differences observed in protease activities of meagre larvae during the samplin...

We study complexified Harmonic Oscillator with a position dependent mass, referred to in the present paper as Exotic Oscillator (EO), in arbitrary dimensions. The real space EO has an interesting dynamics: in the equation of motion the full Hamiltonian operator appears in place of the frequency parameter in a Harmonic Oscillator. We reveal some generic features in the constraint structure of th...