Single-molecule FRET is a powerful tool for probing the kinetic mechanism of a complex enzymatic reaction. However, not every reaction intermediate can be identified via a distinct FRET value, making it difficult to fully dissect a multistep reaction pathway. Here, we demonstrate a method using sequential kinetic experiments to differentiate each reaction intermediate by a distinct time sequenc...

Primary reaction pathways for 2-naphthalenyl + acetylene predicted by the kinetic model under experimental conditions in this work.

This study focused on kinetic modeling of a specific type of multistep heterogeneous reaction comprising exothermic and endothermic reaction steps, as exemplified by the practical kinetic analysis of the experimental kinetic curves for the thermal decomposition of molten ammonium dinitramide (ADN). It is known that the thermal decomposition of ADN occurs as a consecutive two step mass-loss proc...

The objective of this work is investigation of the adsorption and decomposition of Reactive Blue 19 from industrial wastewaters using laccase enzyme immobilized on activated carbon powder and fiber as adsorbent. Time, pH, temperature, stirring rate, the amount of the adsorbent, dye initial concentration, solution flow rate in the column and column height were studied as key operating parame...

Kinetic Monte Carlo simulation was applied to investigation of kinetics and mechanism of oxalic acid degradation by direct and heterogeneous catalytic ozonation. La-containing perovskites including LaFeO3, LaNiO3, LaCoO3 and LaMnO3 was studied as catalyst for oxalic acid ozonation. The reaction kinetic mechanisms of each abovementioned catalytic systems has been achieved. The rate constants val...

To explain the observed dynamics in equilibrium single-molecule measurements of biomolecules, the experimental observable is often chosen as a putative reaction coordinate along which kinetic behavior is presumed to be governed by diffusive dynamics. Here, we invoke the splitting probability as a test of the suitability of such a proposed reaction coordinate. Comparison of the observed splittin...

The steady-state kinetics of the reaction catalyzed by glycogen phosphorylase b from rabbit skeletal muscles has been studied in a wide range of the concentrations of adenosine 5 ′ -monophosphate in the direction of glycogen synthesis under the conditions of saturation of the enzyme by glycogen. The kinetic model has been proposed to describe the bell-shaped dependence of the initial rate of th...

For the quantitative understanding of complex chemical reaction mechanisms, it is, in general, necessary to accurately determine the corresponding free energy surface and to solve the resulting continuous-time reaction rate equations for a continuous state space. For a general (complex) reaction network, it is computationally hard to fulfill these two requirements. However, it is possible to ap...

A two-dimensional single particle finite element model was used to examine the effects of particle fragmental pattern on the average molecular weights, polymerization rate and particle overheating in heterogeneous Ziegler-Natta olefin polymerization. A two-site catalyst kinetic mechanism was employed together with a dynamic two-dimensional molecular species in diffusion-reaction equation. The i...

The kinetics and mechanism of the reaction between iron() and sulfur() was studied at high iron() excess at 10.0 and 25.0 C in 1.0 M NaClO4 by the stopped-flow method. The number of absorbing species in this system was determined by matrix rank analysis of time-resolved spectra. The reaction exhibits composite kinetic features which could be explained by considering the reactions of Fe(...