The development of the electrophoretic procedure has led a number of in%-estigators to study the distribution of rat plasma proteins by this method. Rat serum or plasma does not yield the distinct pat,terns that are obtained with human plasma. Moore, Levin, and Leathem (1) could not observe the cr-globulin fraction in normal rat serum dialyzed against phosphate buffer at pH 7.4. After hypophyse...

A simple, sensitive, and specific liquid chromatography tandem mass-spectrometric method was developed and validated for the determination of epimedin B in rat plasma and tissue samples. After being processed with a protein precipitation method, these samples were separated on an Agilent Eclipse XDB-C18 column with an isocratic mobile phase consisting of acetonitrile and 0.1% formic acid aqueou...

Background: Formaldehyde is a chemical that used in fixed tissues and bodies. Formaldehyde during dissection of cadaver, evaporate and be released. Studies have shown that the gas increase endogenous opioids, nitric oxide and cytokines in plasma. These impacts can affect on gonadotropin hormones. The aim of this study was to investigate possible changes in gonadotropin and epithelial cells of t...

The major site(s) of synthesis of plasma fibronectin is unknown. Using the isolated perfused rat liver and anti-rat fibronectin antiserum, we have measured net hepatic synthesis of fibronectin during 10-hr perfusion periods. We calculated the circulating plasma fibronectin pool of a 195-g rat with body surface area of 300 sq cm to be 3.3 mg: net hepatic synthesis of 3.75 +/- 1.0 mg/300 sq cm bo...

The immunochemical technique, first employed in the study of albumin synthesis in chicken liver slices by Peters and Anfmsen (3), has recently been successfully used with rat liver slices by Marsh and Drabkin (4) as well as by Campbell and Stone (5). The present work extends this technique to the problem of the biosynthesis of plasma lipoprotein by rat liver. The liver has been shown to be the ...

Objective: To develop an ultraperformance liquid chromatography (AcquityTM UPLC) method for simple, fast, accurate and simultaneous determination of nitrite and nitrate ions as stable forms of nitric oxide (NO) production in rat plasma. Methods: The ion-pairing AcquityTM UPLC method with reverse phase BEH C18 column was used along with photodiode array (PDA) UV-Vis detector to separate and dete...

PURPOSE To determine the in vitro plasma protein binding, and the in vivo brain, heart and plasma concentrations of ethopropazine (ET) enantiomers in the rat after iv doses. METHODS For in vivo assessment of ET enantiomer concentrations, rats with implanted jugular vein cannulae were injected with 10 mg/kg of (+/-)-ET HCl. At selected times after dosing, rats were sacrificed and heart, brain,...

The hydrolyzing properties of plasma esterases for aspirin were investigated in human plasma and plasma from experimental animals. The observed rates of aspirin hydrolysis were in the following order: rabbit > human > monkey > rat > mouse > dog > minipig. In human, monkey, and dog plasma, aspirin was hydrolyzed by their major hydrolases, paraoxonase (PON), butyrylcholinesterase (BChE), and albu...

There are several lines of evidence which suggest that the liver is a major source of plasma cholesterol esters. First, eviscerated rats cannot maintain a normal concentration of cholesterol esters in the plasma (1); second, when isotopically labeled mevalonic acid is administered to rats, the specific radioactivity of the hepatic cholesterol esters initially exceeds that of the plasma choleste...

The (mRen2)27 transgenic rat [TGR(mRen2)27] is said to have low plasma levels of active renin. We used a direct radioimmunoassay (RIA) for mouse submaxillary renin, as well as an indirect enzyme-kinetic assay based on the generation of angiotensin I with modification of the pH optimum, to measure rat and mouse plasma renin activity (PRA), plasma renin concentration (PRC), and plasma prorenin in...