Campylobacter jejuni is one of the most common causes of bacterial diarrhea worldwide and is the primary bacterial cause of food-borne illness. Adherence to and invasion of epithelial cells are the most important pathogenic mechanisms of Campylobacter diarrhea. Molecular characterization of invasive and noninvasive Campylobacter isolates from children with diarrhea and symptom-free children was...

Rice blast is not only one of the earliest known plant disease but also one of the most widely distributed, occurring in every region of the world where rice is grown. Ten isolates of Pyricularia oryzae causing rice blast disease were evaluated for their morphological, pathogenic, virulence and genetic characterization using RAPD marker. Isolates were classified into three groups based on patho...

Sorghum ranks fifth in worldwide economic importance among cereal crops and is one of the most important summer annual grasses of Pakistan. As it is a very diverse crop, sorghum genetic fingerprinting requires an efficient marker system. We estimated genetic divergence among 29 sorghum (Sorghum bicolor) genotypes, including approved varieties and local and exotic lines collected from different ...

Gene mapping for a Cupressus species is presented for the first time. Two linkage maps for the Mediterranean cypress (Cupressus sempervirens) varieties, C. sempervirens var. horizontalis and C. sempervirens var. pyramidalis, were constructed following the pseudo-testcross mapping strategy and employing RAPD, SCAR and morphological markers. A total of 427 loci (425 RAPDs, two SCARs) repres...

RAPD is a simple dominant marker system widely used in biology. Effectiveness of RAPD can be improved by selecting and redesigning primers whose priming sites occur in target sequence(s) of gene or organism at optimum distance. We developed software that uses sequences of random decamer primers and nucleotide sequence(s) as two input files. It locates the priming sites in input sequences and ge...

BACKGROUND The root of Boehmeria spp (ramie) is a hepatoprotective Chinese herbal medicine. Medicinal properties vary between Boehmeria nivea var. nivea and Boehmeria nivea var. tenacissima, which are local species found in Taiwan. As commercial preparations may use either species, there is a need for a rapid and simple assay to identify variants for quality control. METHODS Four methods were...

Assam of the northeastern region of India is unique in terms of its rich biodiversity and multiple ethnicity of its people. The impact of the resultant socio-religiocultural diversity is also reflected in the diverse traditional ways of silkworm farming. We report the genetic diversity of Persea bombycina “Som” from different locations of Goalpara district of Assam, India, where random amplifie...

Random amplified microsatellite polymorphism (RAMP) is a PCR-based marker which uses a combination of two classes of markers: Simple sequence repeat (SSR) and Random amplified DNA polymorphism (RAPD) markers. RAMP has been demonstrated to be a potentially valuable molecular marker for the study of genetic relationships in cultivated plant species. The objective of this study was to optimize the...

The present study investigated and identified genetic diversity between native White chicken lines two commercial Broiler (Rose) Layer (Isa Brown) breeds using RAPD markers a sequencing technique. All primers applied produced 151 scorable bands with percentage polymorphic loci of 54.93 % within populations, as per the results marker. maximum amplified fragment by primer OPC-11 was 22 fewest OPA...

Almond is considered the most common and essential nut grown in rainfed area. It has many species wildly distributed Iraqi Kurdistan region, which are called Chaqala by local people. To understand improve characteristics of cultivated almonds, a comprehensive study wild almonds terms population structure genetic diversity needed to transfer new traits into domesticated almonds. So, twelve diffe...