The spectroscopic characterization of biomolecular structures requires nanometer spatial resolution and chemical specificity. We perform full spatio-spectral imaging of dried purple membrane patches purified from Halobacterium salinarum with infrared vibrational scattering-type scanning near-field optical microscopy (s-SNOM). Using near-field spectral phase contrast based on the Amide I resonan...

We are testing a strategy for creating three-dimensional crystals of integral membrane proteins which involves the addition of a large soluble domain to the membrane protein to provide crystallization contacts. As a test of this strategy we designed a fusion between the membrane protein bacteriorhodopsin (BR) and the catalytic subunit of aspartyl transcarbamylase from Escherichia coli. The fusi...

We report that atomic force microscopy based high-speed nanomechanical analysis can identify components of complex heterogeneous synthetic and biological membranes from the measured spectrum of nanomechanical properties. We have investigated phase separated ternary lipid bilayers and purple membranes of Halobacterium salinarum. The nanomechanical spectra recorded on these samples identify all m...

Divalent cations are involved in the function of bacteriorhodopsin (bR) as a light-driven proton pump. If cations are removed from purple membranes they become blue. Divalent cations such as Ca2+ or Pb2+ or trivalent ions, can be stoichiometrically titrated back on to these deionized membranes. The color transitions as a function of ion concentration for Ca2+ or Pb2+ are precisely comparable an...

Since membrane potential regulates the activity of nerves, muscle, and endocrine cells, and many drugs alter the membrane potential, an understanding of the general concepts for the regulation of membrane potential is important. Yet, membrane potentials and ion gradients are often tough areas for students to understand. Analogies can be helpful; for example, Cardozo (2) recently provided a spri...