mycobacterium tuberculosis (h37rv strain) was used in this study. the bacterial cells were disintegrated by sonication. the separation and characterization of the soluble molecules were attempted by various techniques including gel filtration, ion exchange chromatographies and polyacrylamide gel electrophoresis, using sds and 2me. eight protein molecules with molecular weights ranging from 6.3 ...

the aim of this study was production and partial purification of α-amylase enzyme by bacillus licheniformis. b. licheniformis was allowed to grow in broth culture for purpose of inducing α-amylase enzyme. optimal conditions for amylase production by b. licheniformis are incubation period of 120 h, temperature of 37 °c and ph 7.0. the α-amylase enzyme was purified by ion exchange chromatography ...

phenylalanine dehydrogenase (phedh; ec 1.4.1.20) is an important enzyme of amino acid dehydrogenases family that increasingly used as a valuable biocatalyst in neonatal screening kits and synthesis of l-phenylalanine. the goal of this literature was to find a suitable purification method for recombinant bacillus badius phedh by practical comparison between chromatographic and polyvinyl pyrrolid...

two peroxidases (ec. 1.11.1.7), pod i and pod ii were purified from the roots of cultivatedraphanus sativus l. var. cicil by one step ion-exchange chromatography after fractionation by acetone. the molecular weight of these enzymes were 43000 and 41000 daltons and rz 1.2 and 2.0 for pod i and pod ii, respectively. both enzymes consisted of a single polypeptide chain on sds-page. the maximum act...

transferrin acts as a vehicle for iron transportation between the absorption, storage and utilization sites in the body and, besides iron, its two highaffinity iron-binding site are capable of binding to 29 other elements. in physiological and pathophysiological conditions, transferrin’s properties are different and close attention to these properties is required. for further investigation for ...

Taxonomic composition and seasonal dynamics of holoplankton (mesoplankton) have been studied in the seaports Caucasus Taman 2017-2021. Holoplankton communities are characterized by a small number dominant species. Organisms with detritus nutrition strategy, such as rotifers, developed along copepodsin semi – closed port limited water exchange, low transparency higher trophic status. The total a...

Objective(s): We previously conducted an in silico research on the interactions between the ribosome display-selected single chain variable fragment (scFv) and factor H binding protein (fHbp) of Neisseria meningitidis. We found that heavy chain variable (VH) fragment of this scFv had considerable affinity to fHbp. These results led us to evaluate the ability of this sm...

Available online 1 April 2009

Treatment of domestic wastewater, at both the village and urban level, remains a problem in most developing countries. This is a review paper illustrating the use of duckweed-based wastewater treatment systems that provide genuine solutions to these problems. They are inexpensive to install as well as to operate and maintain. They do not require expensive technologies. Therefore, this review pa...

A number of years ago the Green Chemistry Centre of Excellence at York discovered new high surface area forms of polysaccharides. Further study of these materials has found that after controlled thermal treatment they exhibit remarkable thermal properties, which have enabled us to make an entirely new class of materials with properties ranging from starch-like to carbon-like (see figure 1). Tra...