Studies on the physiological role of heme oxygenase (HO) require an inhibitor that will selectively inhibit HO activity without inhibiting the activity of either nitric oxide synthase (NOS) or soluble guanylyl cyclase (sGC). The objective of this study was to test a series of metalloporphyrins that have previously been shown to inhibit HO activity, for their ability to inhibit HO without inhibi...

BACKGROUND Normal and cancerous tissues have distinct auto-fluorescence properties because of differences in their biophysical and biochemical agents. Scientific evidences related to diagnostic fluorescence imaging for detection of oral precancerous and cancerous lesions are very limited. OBJECTIVES The aim of this study was to find out potential relationships between serum, salivary and tiss...

1. A preparation of completely haemolysed erythrocytes from the blood ofdomestic fowls, which actively incorporates [Ox-14C]glycine into haem, is described. 2. The effects of time and of glycine concentration on the rate of synthesis were studied. 3. Stimulation of the synthesis up to sixfold was obtained with some preparations of boiled yeast extract. 2:4-Dinitrophenol inhibited the synthesis ...

In plants, the accumulation of the chlorophyll precursor Mg-protoporphyrin IX (Mg-Proto) in the plastid regulates the expression of a number of nuclear genes with functions related to photosynthesis. Analysis of the plastid-to-nucleus signaling activity of Mg-Proto in Arabidopsis thaliana led to the discovery of GUN4, a novel porphyrin-binding protein that also dramatically enhances the activit...

T HE VARIOUS anemias have been studied and classified clinically, morphologically, therapeutically and to a less extent etiologically, but comparatively few chemical studies have been made. Investigation of the chemical changes accompanying the various types of anemia offers a new approach to their study and gives rise to the hope that as the specific defects are elucidated their correction wil...

Fluorescence spectra of protoporphyrin bound to its most affinitive site on human serum albumin, bound to human haemopexin and dissolved in human plasma reveal that, when present in plasma, at least 90% of this porphyrin is bound to albumin. Human serum albumin binds protoporphyrin with an affinity KA = 3 X 10(9)M-1 in phosphate-buffered saline. The affinity of haemopexin for protoporphyrin is ...

Oxidative liver injury during steatohepatitis results in aggregation and transglutaminase-2 (TG2)-mediated crosslinking of the keratin cytoplasmic intermediate filament proteins (IFs) to form Mallory-Denk body (MDB) inclusions. The effect of liver injury on lamin nuclear IFs is unknown, though lamin mutations in several human diseases result in lamin disorganization and nuclear shape changes. W...