Corybas carsei is one of New Zealand’s most endangered orchids, with a single remaining population in the Whangamarino Wetland (North Island). Although orchid mycorrhizae have proved critical aspect conservation globally, until now mycorrhizal fungi C. not been identified or utilised for conservation. This study investigated fungal associates using three culture-based approaches: grown directly...

This first-attempt study used microtome-based methods to generate a thin cell layer culture for the micropropagation of Phal. Hwafeng Redjewel × New Cinderella. Protocorms were embedded in various agarose concentrations (8–12%, w/v) and dried from 1 8 h before sectioning with microtome. Optimal conditions slicing sections 100 300 μm achieved when protocorms at 10% (w/v) 4 under laminar flow, ha...

Synthetic seeds were prepared in Malaxis acuminata by encapsulating shoot tip derived protocorm-like bodies (PLBs; 0.2-0.5 cm) a hydrated gel capsule composed of calcium alginate matrix (sodium alginate; 3.5% and chloride; 75 mM) liquid Murashige Skoog, 1962 (MS) medium. varied physical features with the concentration gelling agent chloride. In vitro germination, optimum response was achieved M...

The compatibility of mycorrhizal fungi with the early growth stage orchids is essential for their growth. In this study, and promotion effects 14 Tulasnella strains from different hosts were studied by co-culturing them protocorms Dendrobium crepidatum, which has high ornamental economic value in China. ITS–LSU–SSU–TEF combined sequence analysis divided into three clades belonging to calospora ...