Phenotypic screening is a powerful technique that allowed the discovery of antimicrobials to fight infectious diseases considered deadly less than century ago. In high throughput phenotypic assays, thousands compounds are tested for their capacity inhibit microbial growth in-vitro . After an active compound found, identifying molecular target next step. Knowing specific key understanding its me...

Equine recurrent uveitis (ERU) is an autoimmune disease that occurs with a high prevalence (10%) in horses. ERU represents the only reliable spontaneous model for human autoimmune uveitis. We already identified and characterized novel autoantigens (malate dehydrogenase, recoverin, CRALBP) by analyzing the autoantibody-binding pattern of horses affected by spontaneous recurrent uveitis (ERU) to ...

This article reviews recent developments in droplet microfluidics enabling high-throughput single-cell analysis. Five key aspects in this field are included in this review: (1) prototype demonstration of single-cell encapsulation in microfluidic droplets; (2) technical improvements of single-cell encapsulation in microfluidic droplets; (3) microfluidic droplets enabling single-cell proteomic an...

The dismal prognosis of pancreatic cancer has motivated research into identifying non-invasive ‘liquid biopsy’ biomarkers for early detection when treatment is most effective. Recently, exosomes—nanoscale membranous vesicles shed from tumour cells and which can be found circulating in the blood (and most bodily fluids)—have been discovered to contain a wealth of proteomic and genetic informatio...

DNA microarrays and, more recently, protein microarrays, have become important tools for high-throughput genomic and proteomic studies. Transfected cell microarrays are a complementary technique in which array features comprise clusters of cells overexpressing defined cDNAs. Complementary DNAs cloned in expression vectors are printed on microscope slides, which become living arrays after the ad...

Newly translated tubulin molecules undergo a series of complex interactions with nascent chain-binding chaperones, including prefoldin (PFD) and chaperonin-containing TCP-1 (CCT). By screening for oryzalin hypersensitivity, we identified several mutants of Arabidopsis that have lesions in PFD subunits. The pfd6-1 mutant exhibits a range of microtubule defects, including hypersensitivity to oryz...