UNLABELLED Mammalian target of rapamycin (mTOR) is a key regulator of cell growth, proliferation and angiogenesis. mTOR signaling is frequently hyper activated in a broad spectrum of human cancers thereby making it a potential drug target. The current drugs available have been successful in inhibiting the mTOR signaling, nevertheless, show low oral bioavailability and suboptimal solubility. Con...

Protein concentrates and by-products produced from ground wheat were analyzed for amino acid composition, protein, starch, fat, fiber, ash, and various neutral carbohydrates. The concentrates contained from 83 to 92% protein (nitrogen X 5.7), 2.1 to 2.6 g of lysine, 2.5 to 3.5 g of total sulfur amino acids per 16 g of nitrogen, 1.5 to 7.4% fat, 0.9% ash, and from 1.8 to 3.9% total carbohydrate....

Bacteria are the predominant source for producing recombinant proteins but while many exogenous proteins are expressed, only a fraction of those are soluble. We have found that a new actin regulatory enzyme Mical is poorly soluble when expressed in bacteria but the use of a Nus fusion protein tag greatly increases its solubility. However, available vectors containing a Nus tag have been enginee...

Protein crystals usually grow at a preferable temperature which is however not known for a new protein. This paper reports a new approach for determination of favorable crystallization temperature, which can be adopted to facilitate the crystallization screening process. By taking advantage of the correlation between the temperature dependence of the second virial coefficient (B(22)) and the so...

In this study, the effects of limited hydrolysis and/or high-pressure homogenization (HPH) treatment in acid conditions on the functional properties of oyster protein isolates (OPI) were studied. Protein solubility, surface hydrophobicity, particle size distribution, zeta potential, foaming, and emulsifying properties were evaluated. The results showed that acid treatment led to the dissociatio...

The production of correctly folded protein in Escherichia coli is often challenging because of aggregation of the overexpressed protein into inclusion bodies. Although a number of general and protein-specific techniques are available, their effectiveness varies widely. We report a novel method for enhancing the solubility of overexpressed proteins. Presence of a dipeptide, glycylglycine, in the...

Expression of eukaryotic proteins in E. coli often results in their aggregation. Proper folding and solubility of therapeutical proteins are the pre-requisite for their bioactivity. This is not achieved in cytoplasmic expression in E. coli because of the absence of disulfide bonds formation. A novel expression/secretion vector was constructed which exploited β-lactamase signal sequence to trans...

Summary Batter stabilisation presents a great challenge for gluten‐free (GF) bread, as CO 2 is released during bread‐making process, resulting in small, dense and crumbly breads. Apart from starch, protein plays crucial role gas cell stabilisation. This study aims to assess the effect of non‐gluten different sources (plant animal) on GF batter's rheological behaviour (pasting properties, rheolo...

Ammonium sulfate, as well as potassium phosphate, can be used to measure solubility differences between hemoglobin S and hemoglobin A. In addation, the solubility of deoxyhemoglobin C(Harlem) in 1.96 M phosphate has a markedly different temperature dependence from that of deoxyhemoglobin S. This observation indicates that the solubility measurement is quite sensitive to changes in protein struc...

Structure determination of ligands bound to their target proteins using X-ray crystallography is an important part in the drug discovery process. However, this is not always easy to achieve, especially in cases where the affinity is poor and the solubility of the ligand in the crystallization condition is low. The aim of this project was to investigate a possible correlation between the solubil...