Experiment design and execution is a central activity in the natural sciences. The SeqER system provides a general architecture for the integration of automated planning techniques with a variety of domain knowledge in order to plan scientific experiments. These planning techniques include rule-based methods and, especially, the use of derivational analogy. Derivational analogy allows planning ...

A new strategy for construction of synthetic gene encoding human basic fibroblast growth factor comprising DNA annealing-ligation and augmentation by polymerase chain reaction was introduced. The sequence of the gene and corresponding amino acid chain were modified in order to increase stability of the protein. First, 300 bp and 160 bp fragments of the gene were assembled from 18 oligonucleotid...

Background: Promyelocytic leukemia protein (PML) is a tumor suppressor protein that is involved in myeloid cell differentiation in response to retinoic acid (RA). In addition, RA acts as a natural morphogen in neural development. Objectives: This study aimed to examine PML gene expression in different stages of in vitro neural differentiation of NT2 cells, and to investigate the possible role o...

Cloning and sequencing of a lipase gene from an indigenous Bacillus pumilus, strain F3, revealed an open-reading frame of 648 nucleotides predicted to encode a protein of 215 residues. Sequence analysis showed that F3 lipase contained a signal peptide composed of 34 amino acids with an H domain of 18 residues. A tat-like motif was found in the signal peptide similar to some other Bacillus pumil...

objective(s): up to now, many researches have been performed to improve the antitumoral effect of melanoma differentiation-associated gene-7 (mda-7) protein. the purpose of our research was to construct 3 expression vectors producing mda-7 in fusion with rgd (arginine-glycine-aspartic acid) peptide and evaluate their expression.     materials and methods: mda-7 gene with two different rgd seque...

b a ckground: zoonotic cutaneous leishmaniasis (zcl) is endemic in many parts of iran. recently its incidence isconsiderable in different parts of jahrom district, in fars province, southern iran. the aims of our study were to in- vestigate the prevalence of leishmania infection, and identify and characterize the   leishmania species present, among the rodents by molecular methods in a new ende...